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NEB/SalI-HF®/R3138S/10,000 units188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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产品资料

NEB/SalI-HF®/R3138S/10,000 units

Description:

  
HighFidelity(HF®)RestrictionEnzymeshave100%activityinCutSmart™Buffer;single-buffersimplicitymeansmorestraightforwardandstreamlinedsampleprocessing.HFenzymesalsoexhibitdramaticallyreducedstaractivity.HFenzymesareallTime-Saver™qualifiedandcanthereforecutsubstrateDNAin5-15withtheflexibilitytodigestovernightwithoutdegradationtoDNA.Engineeredwithperformanceinmind,HFrestrictionenzymesarefullyactiveunderabroaderrangeofconditions,minimizingoff-targetproducts,whileofferingflexibilityinexperimentaldesign.

NEBextensivelyperformsqualitycontrolsonallstandardandhigh-fidelity(HF®)restrictionenzymes.ExamplesofnucleasecontaminationstudiesforsomeofourHFrestrictionenzymesareshownbelow.

RestrictionEnzymeCompetitorStudy:NucleaseContamination


EcoRI,NotI,andBamHIfrommultiplesuppliersweretestedinreactionscontainingafluorescentlabeledsinglestranded,doublestrandedblunt,3’overhangor5’overhangcontainingoligonucleotides.Thepercentdegradationisdeterminedbycapillaryelectrophoresisandpeakanalysis.Theresolutionisatthesinglenucleotidelevel.

ProductSource

An E.coli strainthatcarriestheclonedandmodifiedSalIgenefrom Streptomycesalbus G(ATCC49789)

ReagentsSupplied

Thefollowingreagentsaresuppliedwiththisproduct:

Storeat(°C)Concentration
CutSmart®Buffer-2010X
GelLoADIngDye,Purple(6X)256X
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