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TheLyraDirectHSV1+2/VZVAssayisamultiplexinvitrotestthatdetectsanddifferentiates Herpessimplexvirustype1,Herpessimplexvirustype2,andVaricella-zostervirusnucleicacidsisolatedandpurifiedfromcutaneousormucocutaneousswabspecimensobtainedfromsymptomaticpatients.
Amultiplexreal-timePCRreactioniscarriedoutunderoptimizedconditionsinasingletubegeneratingampliconsforHSV-1,HSV-2,VZV,andtheProcessControl(PRC).IdentificationofHSV-1,HSV-2,VZV,andthePRCoccursbytheuseoftarget-specificprimersandfluorescent-labeledprobesthathybridizetoconservedregionsinthegenomesofHSV-1,HSV-2,VZVandthePRC.
TheLyraDirectHSV1+2/VZVAssayhasone-stepreagentset-upandathree-stepsampleprep.Testresultsareavailableinlessthan70minutes.TheLyraDirectHSV1+2/VZV AssayisavailableforuseonAppliedBiosystems®7500FastDx,QuantStudio™DxReal-TimePCRInstrument,andCepheid®SmartCycler®II.
TheLyraDirectHSV1+2/VZVAssayisoneinagrowingmenuofPCRtestsandisusedasanaidinthedifferentialdiagnosisofHerpessimplexvirus1and2(HSV-1andHSV-2)andVaricella-zostervirus(VZV)alsoknownasHumanherpesvirus3.VZVisthecausativeagentforchickenpoxandshingles.
| Feature | Benefit |
|---|---|
One-stepreagentset-up | RehydrationsolutionissimplyaddedtothelyophilizedMasterMix |
Speed | Resultsinlessthan60minutes |
Easytraining | Simplifiedanduniformworkflowwithstandardpipettingvolumes |
Refrigeratedstorage | Nofreezerneeded.2°Cto8°Cstorage |
Longshelflife | 2yearsfromdateofmanufacture |
Roomtemperaturesetup | Noiceorcoolingblockrequired |
FlexIBLe96-testformat | Kitincludesvialsof12x8MasterMix,RehydrationSolutionandliquidProcessControls.Samplepreparationkitincludesallconsumables |
| ProductSpecName | ProductSpecData |
|---|---|
Sampletype | Cutaneousormucocutaneousswabspecimens |
Timetoresults | Lessthan60minutes |
Reagentstorageconditions | 2°Cto8°C |
Controlsstorageconditions | 2°Cto8°C |
Samplevolumeneededforextraction | 100µL |
Purifiednucleicacidvolumeneededtotesting | 5µL |
Sensitivity* | Cutaneous:HSV-1100.0%,HSV-297.1%,VZV96.3%;Mucocutaneous:HSV-195.1%,HSV-297.9%,VZV100.0% |
Specificity* | Cutaneous:HSV-198.8%,HSV-296.7%,VZV96.4%;Mucocutaneous:HSV-198.2%,HSV-297.1%,VZV99.3% |
Shelflife | 24months |
*DataforAppliedBiosystems™7500FastDx. RefertoPackageInsertforadditionalperformanceclaims.
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