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TheLyraRSV+hMPVAssayisamultiplexreal-timePCRassayforthequalitativedetectionandidentificationofrespiratorysyncytialvirus(RSV)andhumanmetapneumovirus(hMPV)viralRNAextractedfromnasalswabs,nasopharyngealswabs,andnasalaspirate/washspecimens.ThisrealtimeRSV+hMPVtestdetectsRSVandhMPVandisabletodifferentiatebetweenthem.
Amultiplexreal-timeRT-PCRreactioniscarriedoutunderoptimizedconditionsinasingletubegeneratingampliconsforRSV,hMPVandtheProcessControl(PRC).IdentificationofRSVandhMPVandthePRCoccursbytheuseoftarget-specificprimersandfluorescent-labeledprobesthathybridizetoconservedregionsintheNS2andpolymerasegenesofRSVandpolymerasegeneofhMPVandthePRC.
TheLyraRSV+hMPVAssayhasaone-stepreagentsetup.Testresultsareavailableinlessthan75minutesafterextraction.LyraRSV+hMPVRT-PCRisavailableforuseontheAppliedBiosystems®7500FastDx,QuantStudioTMDxReal-TimePCRInstrumentandCepheid®SmartCycler®II.
TheLyraRSV+hMPVAssayisoneinagrowingmenuoftestsandintendedtoaidinthedifferentialdiagnosisofRSVandhMPVinfectionsinhumans.
| Feature | Benefit |
|---|---|
One-stepreagentset-up | RehydrationsolutionissimplyaddedtothelyophilizedMasterMix |
Speed | Resultsinlessthan70 minutesafterextraction |
Easytraining | Simplifiedanduniformworkflowwithstandardpipettingvolumes |
Refrigeratedstorage | Nofreezerneeded.2°Cto8°Cstorage |
Longshelflife | 2yearsfromdateofmanufacture |
Roomtemperaturesetup | Noiceorcoolingblockrequired |
FlexIBLe96-testformat | Kitincludesvialsof12x8MasterMix,RehydrationSolutionandliquidProcessControls |
| ProductSpecName | ProductSpecData |
|---|---|
Sampletype | Nasalswab,Nasopharyngealswab |
Timetoresults | Lessthan75minutesafterextraction |
Reagentstorageconditions | 2°Cto8°C |
Controlsstorageconditions | 2°Cto8°C |
Sensitivity* | RSV:98.6% |
Specificity* | RSV:96.8% |
PositivePercentAgreement* | hMPV:98.0% |
NegativePercentAgreement* | hMPV:98.9% |
LOD | RSVA:6.29E-01;RSVB:7.5E-01;hMPV-A1:1.7E+01;hMPV-A2:2.91E+00;hMPV-B1:1.05E+00;hMPV-B2:2.25E+00 |
Samplevolumeneededforextraction | 180µL |
Purifiednucleicacidvolumeneededtotest | 5µL |
Shelflife | 24months |
*DataforAppliedBiosystems™7500FastDx. RefertoPackageInsertforadditionalperformanceclaims.
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