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Bpsbioscience/FBXL10 (KDM2B) Chemiluminescent Assay Kit/50157/96 reactions

Description:
TheFBXL10ChemiluminescentAssayKitisdesignedtomeasureFBXL10activityforscreeningandprofilingapplications.FBXL10,alsoknownasKDM2B,JHDM1B,andCXXC2,isahistonelysinedemethylasethatexhibitsdemethylationactivitytowardtrimethyllysine4(H3K4me3)anddimethyl-lysine36(H3K36me2)onhistoneH3.ThekeytotheFBXL10ChemiluminescentAssayKitisahighlyspecificantibodythatrecognizesdemethylatedsubstrate.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequiredfordetectionofdemethylaseactivity.First,FBXL10enzymeisincubatedwiththemethylatedH3peptideforonehour.Next,primaryantibodyisadded.Finally,theplateistreatedwithanHRP-labeledsecondaryantibodyfollowedbytheadditionoftheHRPsubstratetoproducechemiluminescencethatcanbemeasuredusingachemiluminescencereader.
Synonym(s):
KDM2B,JHDM1B,CXXC2
SuppliedAs:
TheFBXL10ChemiluminescentAssayKitcomesinaconvenient96-wellstripplateformat,precoatedwithmethylatedhistoneH3peptidesubstrate,primaryantibody,secondaryHRP-labeledantibody,demethylaseassaybuffer,andpurifiedFBXL10for100enzyme
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:

COMPONENTS:

Instructionsforuse:
Seeproductdatasheetfordetailedprotocol.
Storage/StABIlity:
Oneyearfromdateofreceiptwhenstoredasdirected.
Application(s):
GreatforstudyingenzymekineticsandHTSapplications.
Reference(s):
Iwase,S.,etal.Cell2007;128(6):1077-1088.
Notes:

ThebufferinthiskitwasreformulatedinMayof2015withadifferentreducingagenttoimproveassayperformance.Theoldformulationcanstillbepurchaseduponspecialrequest.

MATERIALSREQUIREDBUTNOTSUPPLIED:
TBSTbuffer(1xTBS,pH8.0,containing0.05%Tween-20)
LuminometerorfluorescentmicroplatereadercapableofreADIngchemiluminescence
Adjustablemicropipettorandsteriletips
Rotatingorrockerplatform

Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Demethylase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
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