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Bpsbioscience/CECR2, His-tag/31046/100 µg

Species:Human
HostSpecies:E.coli
MW:14.4kDa
Genbank#:NM_031413
Tag(s):N-terminalHis-tag
a.a:430-543
Description:
HumanBromodomainCECR2(cateyesyndromechromosomeregion,candidate2),GenBankAccessionNo.NM_031413,a.a.430-543correspondingtosinglebromodomainwithN-terminalHis-tag,MW=14.4kDa,expressedinanE.coliexpressionsystem.
UniProt
Q9BXF3
Synonym(s):
CECR2,cateyesyndromechromosomeregion,candidate2
AssayConditions:

CeCR2wasincubatedwithvariousamountsofBETligandinassaybufferina10µlreactionfor30minatRT.ReactionswithH41-21[B]werepreparedinparallelascontrol.AlphaLISA®®Niacceptorbeads(1:250)wereaddedandincubationcontinuedfor30minmore.Finally,StreptavidinDonorbeads(1:125)wereaddedandmixturewasincubatedfor10minatRTbeforeA-countsweremeasured.

Formulation:
40mMTrispH8,110mMNaCl,2.2mMKCl,0.04%Tween-20,and20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofbromodomainbindingassays,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.ChenJ,etal.,IntJDevBiol.2010;54(5):925-9.
2.2.LeeSK,etal.,MolCells.2012Jul;34(1):85-91.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Bromodomain
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
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