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Bpsbioscience/Human Interleukin-29/90187-B/20 µg

Species:Human
HostSpecies:E.coli
MW:20kDa
Genbank#:Q8IU54
a.a:24–200
Background:
TheIFN-lamBDagenefamilyiscomposedofthreedistinctgenes:IFN-lambda1(IL-29),IFN-lambda2(IL-28A),andIFN-lambda3(IL-28B).IFN-lambdaproteinsexhibitonlyweakhomologytoIL-10,but,likeIL-10,theyalsousetheIL-10R2chainasacomponentoftheirreceptorcomplex.TheIFN-receptorcomplexconsistsoftheuniqueligand-bindingchainIFN-R.TheIFN-λ1geneisfoundexclusivelyinprimategenomesandis81%identicaltoIFN-λ2/3.IFN-λs,whicharenowcollectivelyreferredtoastypeIIIIFNs,exhibitseveralfeaturesincommonwithtypeIIFNs,suchasantiviral,antiproliferative,andantitumoractivities.
Description:
RecombinantInterleukin-29(IFN-lambda)isadisulfide-linkedmonomerproteinconsistingof178aminoacidresidues,andmigratesasanapproximately20kDaproteinundernon-reducingandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodingmurineInterleukin-29maturechainwasexpressedinE.coli.
UniProt
Q8IU54
Purity:
≥95%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BIOLOGicalActivity:
TheED50isdeterminedinananti-viralassayusinghumanHepG2cellsinfectedwithEMCVistypically1-5ng/ml.
Formulation:
Lyophilizedfrom0.2µmfilteredsolutionin130mMNaCl,20mMphosphatebuffer,pH7.5.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:

Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.

Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
TSKPTTTGKGCHIGRFKSLSPQELASFKKARDALEESLKLKNWSCSSPVFPGNWDLRLLQVRERPVALEAELALTLKVLEAAAGPALEDVLDQPLHTLHHILSQLQACIQPQPTAGPRPRGRLHHWLHRLQEAPKKESAGCLEASVTFNLFRLLTRDLKYVADGNLCLRTSTHPEST
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
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