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Bpsbioscience/FBXL10 (KDM2B, JHDM1B), FLAG-tag/50120/20 µg

Species:Human
HostSpecies:Sf9insectcells
MW:75kDa
Genbank#:NM_032590
Tag(s):C-terminalFLAG-tag
a.a:1–650
Description:
HumanF-BoxProtein10(FBXL10,alsoknownasKDM2B,JHDM1B,CXXC2,andHistone-H3K36demethylase1B),GenBankAccessionNo.NM_032590,a.a.1-650,withC-terminalFLAG-tag,MW=75kDa,expressedinSf9cellsviaabaculovirusexpressionsystem.
UniProt
Q8NHM5
Synonym(s):
F-BoxProtein10,FBXL10,KDM2B,JHDM1B,CXXC2,Histone-H3K36demethylase1B
Purity:
≥83%byHPLC
AssayConditions:
Enzymewasincubatedwith0.4μMsubstrateinHEPES-containingJMJassaybufferSAandSA-(withoutKG)ina10-μlreactionfor180minat30˚C.5μlofAnti-RabbitAlphaLISA®acceptorbeads(1:250)and5μlofrabbitpolyclonalantibody(1:200)wereaddedandincubationcontinuedfor30min.StreptavidinDonorbeads(1:125)wereadded(10μl)andmixturewasfurtherincubatedfor10minatRTbeforeAcountsweremeasured.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and80µg/mlFLAGpeptide
Format:
Aqueousbuffersolution
Storage/StABIlity:

>6monthsat–80°C.

Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Tanaka,Y.,etal.(2010).EMBOJ.29(9):1510-22.
2.Blackledge,N.P.,etal.(2010).Mol.Cell.38(2):179-90.3.Frescas,D.,etal.(2008).CellCycle7(22):3539-47.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Demethylase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
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