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Bpsbioscience/HSP90β N-Terminal Domain Assay Kit/50294/96 reactions

Background:

Hsp90βisamolecularchaperonewithessentialfunctionsinmaintainingtransformation.InhibitionofHsp90βfunctionshasbeenshowntoplayaroleintumOrigenesisanddiseaseprogression.

Description:
TheHSP90βN-TerminalDomainAssayKitisdesignedforidentificationofHSP90βinhibitorsusingfluorescencepolarization.TheassayisbasedonthecompetitionoffluorescentlylabeledgeldanamycinforbindingtopurifiedrecombinantHSP90β.ThekeytotheHSP90βAssayKitisthefluorescentlylabeledgeldanamycin.Usingthiskit,onlyonesimplesteponamicrotiterplateisrequiredforHSP90βreactions.ThefluorescentlylabeledgeldanamycinisincubatedwithasamplecontainingHSP90βenzymetoproduceachangeinfluorescentpolarizationthatcanthenbemeasuredusingafluorescencereader.
UniProt
P08238
Synonym(s):
HSP90b,HeatShockProtein90b,HSP90beta,HSP90β
SuppliedAs:
TheHSP90βinhibitorscreeningassaykitcomesinaconvenient96-wellformat,withpurifiedHSP90βenzyme,fluorescentlylabeledgeldanamycinandHSP90assaybufferfor96enzymereactions.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:
Instructionsforuse:
Seedatasheetfordetailedprotocol.
Storage/StABIlity:

Stableforatleast12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.

Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):

1.Kim,J.,etal.,JBiomolScreening.2004,9(5):375-381.
2.Howes,R.,etal.,AnalBiochem.2006,350:202-213.

ApplicationReference:
AnImpermeantGanetespibAnalogInhibitsExtracellularHsp90-MediatedCancerCellMigrationthatInvolvesLysylOxidase2-likeProtein(2014)

Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
HeatShockProteins
ProductType:
AssayKit
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