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Antibodies-Online/AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (C-Term) antibody/ABIN2778782/100 μL188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Antibodies-Online/AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (C-Term) antibody/ABIN2778782/100 μL

Antigen
AURNABindingProtein/enoyl-CoAHydratase(AUH)
  • 5033405N08Rik
  • wu:fb81b10
  • zgc:101057
  • C77140
  • W91705
  • AURNAbindingprotein/enoyl-CoAhydratase
  • agmatineureohydrolase(agmatinase)
  • AURNAbindingprotein/enoyl-CoenzymeAhydratase
  • AURNAbindingprotein/enoyl-coenzymeAhydratase
  • Auh
  • Agmat
  • auh
  • AUH
Epitope
C-Term
6N-Term
5C-Term
4AA44-135
2partial
1AA228-277
1AA38-87
Reactivity
Cow(Bovine),Dog(Canine),GuineaPig,Horse(Equine),Human,Mouse(Murine),Rabbit,Rat(Rattus),Yeast
29Human
17Mouse(Murine)
14Rat(Rattus)
3Dog(Canine)
2Cow(Bovine)
2Pig(Porcine)
2Rabbit
1Horse(Equine)
1Monkey
Host
Rabbit
27Rabbit
4Mouse
Clonality
Polyclonal
Application
Immunohistochemistry(IHC),WesternBlotting(WB)
28WesternBlotting(WB)
14Immunohistochemistry
(IHC)
9ELISA
6Immunohistochemistry
(Paraffin-embedded
Sections)(IHC(p))
Options
Bulkdiscount
Supplier
SupplierProductNo.
ImmunogenTheimmunogenisasyntheticpeptidedirectedtowardstheCterminalregionofhumanAUH
BlockingPeptideBlockingpeptideforthisproductavailable:ABIN973655
SequenceIGMSLAKELIFSARVLDGKEAKAVGLISHVLEQNQEGDAAYRKALDLARE
PredictedReactivityCow:100%,Dog:100%,GuineaPig:93%,Horse:100%,Human:100%,Mouse:100%,Rabbit:100%,Rat:100%,Yeast:75%
CharacteristicsThisisarabbitpolyclonalantibodyagainstAUH.ItwasvalidatedonWesternBlotandimmunohistochemistry.
PurificationAffinityPurified
AlternativeNameAUH(AUHAntibodyAbstract)
BackgroundAU-specificRNA-bindingenoyl-CoAhydratase(AUH)proteinbindstotheAU-richelement(ARE),acommonelementfoundinthe3"UTRofrapidlydecayingmRNAsuchasc-fos,c-mycandgranulocyte/macrophagecolonystimulatingfactor.AREelementsareinvolvedindirectingRNAtorapiddegradationanddeadenylation.AUHisalsohomologoustoenol-CoAhydratase,anenzymeinvolvedinfattyaciddegradation,andhasbeenshowntohaveintrinsichydrataseenzymaticactivity.AUHisthusabifunctionalchimerabetweenRNAbindingandmetabolicenzymeactivity.ApossIBLesubcellularlocalizationinthemitochondriahasbeendemonstratedforthemousehomologofthisproteinwhichshares92 %identitywiththehumanprotein.IthasbeensuggestedthatAUHmayhaveanovelroleasamitochondriallocatedAU-bindingprotein.HumanAUHisexpressedasasinglemRNAspeciesof1.8kb,andtranslatedasa40- kDaprecursorproteinwhichissubsequentlyprocessedtoa32- kDamatureform.AU-specificRNA-bindingenoyl-CoAhydratase(AUH)proteinbindstotheAU-richelement(ARE),acommonelementfoundinthe3"UTRofrapidlydecayingmRNAsuchasc-fos,c-mycandgranulocyte/macrophagecolonystimulatingfactor.AREelementsareinvolvedindirectingRNAtorapiddegradationanddeadenylation.AUHisalsohomologoustoenol-CoAhydratase,anenzymeinvolvedinfattyaciddegradation,andhasbeenshowntohaveintrinsichydrataseenzymaticactivity.AUHisthusabifunctionalchimerabetweenRNAbindingandmetabolicenzymeactivity.Apossiblesubcellularlocalizationinthemitochondriahasbeendemonstratedforthemousehomologofthisproteinwhichshares92 %identitywiththehumanprotein.IthasbeensuggestedthatAUHmayhaveanovelroleasamitochondriallocatedAU-bindingprotein.HumanAUHisexpressedasasinglemRNAspeciesof1.8kb,andtranslatedasa40- kDaprecursorproteinwhichissubsequentlyprocessedtoa32- kDamatureform.
ProteinInteractionPartner:UBC,AUH,
ProteinSize:339
MolecularWeight37kDa
GeneID549
NCBIAccessionNM_001698,NP_001689
UniProtQ13825
ResearchAreaChromatinandNuclearSignaling,ChromatinBindingProteins,Metabolism,Organelles
ApplicationNotesOptimalworkingdilutionsshouldbedeterminedexperimentallybytheinvestigator.
Comment

Antigensize:339AA

RestrictionsForResearchUseonly
FormatLiquid
ConcentrationLotspecific
BufferLiquid.Purifiedantibodysuppliedin1xPBSbufferwith0.09 %(w/v)sodiumazideand2 %sucrose.
PreservativeSodiumazide
PrecautionofUseThisproductcontainsSodiumazide:aPOISONOUSANDHAZARDOUSSUBSTANCEwhichshouldbehandledbytrainedstaffonly.
HandlingAdviceAvoidrepeatedfreeze-thawcycles.
Storage-20°C
StorageCommentForshorttermuse,storeat2-8°Cupto1week.Forlongtermstorage,storeat-20°Cinsmallaliquotstopreventfreeze-thawcycles.
SupplierImages
Western Blotting (WB) image for anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (C-Term) antibody (ABIN2778782)WBSuggestedAnti-AUHAntibodyTitration:0.2-1ug/ml
ELISATiter:1...
Immunohistochemistry (IHC) image for anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (C-Term) antibody (ABIN2778782)anti-AURNABindingProtein/enoyl-CoAHydratase(AUH)(C-Term)antibody(Image2)
Western Blotting (WB) image for anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (C-Term) antibody (ABIN2778782)WBSuggestedAnti-AUH
AntibodyTitration:0.2-1µg/mLELISATiter:1:.12500