产品资料

Antibodies-Online/Goat anti-Human IgA, IgG, IgM (Whole Molecule) Antibody (Biotin)/ABIN117310/2 mg

Antigen

Epitope

WholeMolecule

Reactivity

Human

Host

Goat

Clonality

Conjugate

Biotin

Application

EnzymeImmunoassay(EIA),Immunohistochemistry(FrozenSections)(IHC(fro)),Immunohistochemistry(Paraffin-embeddedSections)(IHC(p)),WesternBlotting(WB)

Options

Supplier

SupplierProductNo.

Productdetails ReferencesHandlingApplicationDetailsTargetdetailsbacktotop
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Immunogen	HumanIgG,IgAandIgMwholemolecules
Characteristics	MolarRatio:10-20BACmoleculesperGoatIgGmolecule.
Purification	Immunoaffinitychromatography Thisproductwaspreparedfrompolyspecificantiserumbyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeadsfollowedbysolidphaseadsorption(s)toremoveanyunwantedreactivities.

Targetdetails ReferencesHandlingApplicationDetailsbacktotop
Background	Synonyms:HumanIgA,HumanIgG,HumanIgM

ApplicationDetails ReferencesHandlingTargetdetailsbacktotop
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ApplicationNotes	Immunoblotting,ELISA,immunohistochemistry,immunomicroscopyaswellasotherantibodybasedassaysusingstreptavidinoravidinconjugatesrequiringlot-to-lotconsistency.RecommendedDilutions:Thisproducthasbeenassayedagainst1.0 μgofHumanIgGinastandardcaptureELISAusingPeroxidaseConjugatedStreptavidinandABTS(2,2"-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid)asasubstratefor30 minutesatroomtemperature.Aworkingdilutionof1:20,000to1:80,000ofthereconstitutionconcentrationissuggestedforthisproduct. Otherapplicationsnottested. Otimaldilutionsaredependentonconditionsandshouldbedeterminedbytheuser.
Restrictions	ForResearchUseonly

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Reconstitution	Restorewith1.0 mLdeionizedwaterorequivalent.
Concentration	2.0mg/mL
Buffer	0.02MPotassiumPhosphate,0.15MSodiumChloride, pH7.2,0.01 %(w/v)SodiumAzide,10 mg/mLBSA(IgGandProteasefree)
Preservative	Sodiumazide
PrecautionofUse	WARNING:Reagentscontainsodiumazide.Sodiumazideisverytoxicifingestedorinhaled.Avoidcontactwithskin,eyes,orclothing.Weareyeorfaceprotectionwhenhandling.Ifskinoreyecontactoccurs,washwithcopiousamountsofwater.Ifingestedorinhaled,contactaphysicianimmediately.Sodiumazideyieldstoxichydrazoicacidunderacidicconditions.Diluteazide-containingcompoundsinrunningwaterbeforediscardingtoavoidaccumulationofpotentiallyexplosivedepositsinleadorcopperplumbing.
HandlingAdvice	Avoidrepeatedfreezingandthawing.
Storage	4°C/-20°C
StorageComment	Priortoreconstitutionstoreat2-8°C.Followingreconstitutionstoretheantibodyundilutedat2-8°Cforonemonthor(inaliquots)at-28°Cforlonger.

References HandlingApplicationDetailsTargetdetailsbacktotop
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Backgroundpublications	Bayer,Wilchek:"Proteinbiotinylation."in:Methodsinenzymology,Vol.184,pp.138-60,1990(PubMed).

Targetdetails ReferencesHandlingApplicationDetailsbacktotop
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Background	Synonyms:HumanIgA,HumanIgG,HumanIgM