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Agrenvec/Anti-Human Follistatin (IgG) – RF0060/RF0060/100-microg

Description:

 RabbitPolyclonalAntibody(IgG)to Follistatin

Application:

WB,ELISA

Reactivity:

 Human

Conjugate:

 Unconjugated

ELISA:

 Suggestedstartingdilution1/500anti-rabbitIgG-AP(alkalinephosphataseconjugate)isrecommendedassecondaryreagent.

Description: Anti-HumanFollistatinIgGdevelopedinrabbitandpurifiedbyaffinitychromatographyonproteinG(>98%purity).

Immunogen: Highlypure(>97%)recombinanthumanFollistatinexpressedinplants.

Applications:

  • ELISA:Suggestedstartingdilution1/500.Thisantibody,inconjunctionwithcompatIBLesecondaryreagent(anti-rabbitIgG-APconjugate),allowsthedetectionof<10ng/wellofrhumanFollistatin(RF0043).
  • WesternBlot: Suggestedstartingdilution1/500anti-rabbitIgG-AP(alkalinephosphataseconjugate)isrecommendedassecondaryreagent.
RF0060.-WB-Anti-Human-Follistatin-IgG-202x300
Fig1.WesternBlotanalysisofrhumanFollistatinusingAnti-HumanFollistatinIgG(RF0060)
HumanFollistatinproteinwasresolvedbySDS-PAGE,transferredtoaNCmembraneandprobedwithadilution1:1,000ofAnti-HumanFollistatinIgG.Anti-rabbitIgG-AP(alkalinephosphataseconjugate)wasusedassecondaryreagent.Lane1:MWM;Lane2:0.25ugfnonglycosylatedrHumanFollistatinproteinproducedinaprokaryoticexpressionsystem.Lane3:0.25ug ofglycosylatedisoformsofrHFollistatinproducedinaneukaryoticexpressionsystem.
Wherethisantibodyhasnotbeentestedforuseinaparticulartechniquethisnotnecessarilyexcludesitsuseinsuchprocedures.Optimaldilutionconditionsshouldbedeterminedbythefinaluser.Bulksizesareavailableuponrequest.ForR&Dpurposesonly.PurchasermustdeterminethesuitABIlityoftheproduct(s)fortheirparticularuse.
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