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Agrenvec/Anti-Human FLt3 (IgG) – RF0058/RF0058/100-microg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Agrenvec/Anti-Human FLt3 (IgG) – RF0058/RF0058/100-microg

Description:

 RabbitPolyclonalAntibody(IgG)to FLt3

Application:

 WB,ELISA

Reactivity:

 Human

Conjugate:

Unconjugated

ELISA:

 Suggestedstartingdilution1/1,000anti-rabbitIgG-AP(alkalinephosphataseconjugate)isrecommendedassecondaryreagent.

Description: Anti-HumanFLt3IgGisdevelopedinrabbitandpurifiedbyaffinitychromatographyonproteinG(>98%purity).

Immunogen:Highlypure(>97%)recombinanthumanFLt3inexpressedinplants.

Applications:

  • ELISA:Suggestedstartingdilution1/2,000.Thisantibody,inconjunctionwithcompatIBLesecondaryreagent(anti-rabbitIgG-APconjugate),allowsthedetectionof<of10ng/well ofrhumanFLt3 (RF0041).
  • WesternBlot: Suggestedstartingdilution1/1,000anti-rabbitIgG-AP(alkalinephosphataseconjugate)isrecommendedassecondaryreagent.
RF0058.-WB-Anti-Human-FLt3-IgG-238x300
Fig1.WesternBlotanalysisofrhumanFLt3usingAnti-HumanFLt3IgG(RF0058)
HumanFLt3 proteinwasresolvedbySDS-PAGE,transferredtoaNCmembraneandprobedwithadilution1:2,000ofAnti-HumanFLt3IgG. Anti-rabbitIgG-AP(alkalinephosphataseconjugate)wasusedassecondaryreagent.Lane1:MWM;Lane2:0.25ugfnonglycosylatedrHumanFollistatinproteinproducedinaprokaryoticexpressionsystem.Lane3:0.25ugof rHFlt3-ligandproducedintheendospermtissueofbarleygrain. Lane4:0.5ugofglycosylatedisoformsofrHFLt3producedinaneukaryoticexpressionsystem.
Wherethisantibodyhasnotbeentestedforuseinaparticulartechniquethisnotnecessarilyexcludesitsuseinsuchprocedures.Optimaldilutionconditionsshouldbedeterminedbythefinaluser.Bulksizesareavailableuponrequest.ForR&Dpurposesonly.PurchasermustdeterminethesuitABIlityoftheproduct(s)fortheirparticularuse.
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