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Agrenvec/Anti-Human IL12p40 (IgG) – RF0057/RF0057/100-microg

Description:

RabbitPolyclonalAntibody (IgG)to IL12p40

Application:

 WB,ELISA

Reactivity:

 Human

Conjugate:

 Unconjugated

ELISA:

Suggestedstartingdilution1/500. Thisantibody,inconjunctionwithcompatIBLesecondaryreagents(anti-rabbitIgG-APconjugate),allowsthedetectionof <10ng/wellofrhumanIL12p40(RF0031).

WesternBlot:

 Suggestedstartingdilution1/1,000.Anti-rabbitIgG-AP(alkalinephosphataseconjugate)isrecommendedassecondaryreagent.

Description: Anti-HumanIL12p40IgGisdevelopedinrabbitandpurifiedbyaffinitychromatographyonproteinG(>98%purity).

Immunogen: Highlypure(>97%)recombinanthumanIL12p40 expressedinplants.

Applications:

  • ELISA: Suggestedstartingdilution1/500. Thisantibody,inconjunctionwithcompatiblesecondaryreagents(anti-rabbitIgG-APconjugate),allowsthedetectionof <10ng/wellofrhumanIL12p40(RF0031).
  • WesternBlot: Suggestedstartingdilution1/1,000.Anti-rabbitIgG-AP(alkalinephosphataseconjugate)isrecommendedassecondaryreagent.
 RF0057-WB-Anti-Human-IL12p40-IgG-245x300
Fig1.WesternBlotanalysisofrhumanIL12p40usingAnti-HumanIL12p40IgG(RF0057)
HumanIL12p40proteinwasresolvedbySDS-PAGE,transferredtoaNCmembraneandprobedwithadilution1:1,000ofAnti-HumanIL12p40IgG.Anti-rabbitIgG-AP(alkalinephosphataseconjugate)wasusedassecondaryreagent.Lane1:MWM;Lane2:0.25ugfnonglycosylatedrHumanIL-12p40proteinproducedinaprokaryoticexpressionsystem.Lane3:0.25ug ofglycosylatedisoformsofrHIL-12p40producedinaneukaryoticexpressionsystem.
Wherethisantibodyhasnotbeentestedforuseinaparticulartechniquethisnotnecessarilyexcludesitsuseinsuchprocedures.Optimaldilutionconditionsshouldbedeterminedbythefinaluser.Bulksizesareavailableuponrequest.ForR&Dpurposesonly.PurchasermustdeterminethesuitABIlityoftheproduct(s)fortheirparticularuse.
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