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Agrenvec/Anti-Human GM-CSF (IgG) – RF0097/RF0097/100-microg

Description:

RabbitPolyclonalAntibody(IgG)to GM-CSF

Application:

 WB,ELISA

Reactivity:

 Human

Conjugate:

 Unconjugated

Description: Anti-HumanGM-CSFIgGisdevelopedinrabbitandpurifiedbyaffinitychromatographyonproteinG(>98%purity).

Immunogen: Highlypure(>97%)recombinanthuman GM-CSFexpressedinplants.

Applications:

  • ELISA: Suggestedstartingdilution1/4,000.Thisantibody,inconjunctionwithcompatIBLesecondaryreagent(anti-rabbitIgG-APconjugate),allowsthedetectionof>0,5ng/wellofrhumanGM-CSF(RF0036).
  • WesternBlot:Suggestedstartingdilution1/5,000.Anti-rabbitIgG-AP(alkalinephosphataseconjugate)isrecommendedassecondaryreagent.
RF0097.-WB-Anti-Human GM-CSF IgG
‘Fig1.WesternBlotanalysisofrhumanGM-CSFusingAnti-HumanGM-CSFIgG(RF0097)
HumanGM-CSFproteinwasresolvedbySDS-PAGE,transferredtoaNCmembraneandprobedwithadilution1:1,000ofAnti-HumanGM-CSFIgG.Anti-rabbitIgG-AP(alkalinephosphataseconjugate)wasusedassecondaryreagent.Lane1:MWM(kDa);Lane2:0.5ugofrHumanGM-CSFprotein.

Wherethisantibodyhasnotbeentestedforuseinaparticulartechniquethisnotnecessarilyexcludesitsuseinsuchprocedures.

Optimaldilutionconditionsshouldbedeterminedbythefinaluser.Bulksizesareavailableuponrequest.ForR&Dpurposesonly.PurchasermustdeterminethesuitABIlityoftheproduct(s)fortheirparticularuse.
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