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Nkmaxbio/Human 14-3-3 epsilon antibody/100ul/ATGA0124-100ul

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Product Information

Product Type: Monoclonal Antibody

Clone Number: AT4F8

UniProt No.: P62258

NCBI Accession No.: NP_006752

Alternative names: YWHAE, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon, 14-3-3E, HEL2, KCIP-1, MDCR, MDS

Product Information
Product Specification
Data

Product Specification

Host: Mouse

Reacts With: Human

Concentration: 1mg/ml (determined by BCA assay)

Formulation: Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen: Recombinant human14-3-3 epsilon (1-255aa) purified from E. coli

Isotype: IgG2b kappa

Purification: By protein-A affinity chromatography

Applications: ELISA, WB, ICC/IF

Usage: The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Product Information
Product Specification
Data

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of 14-3-3 epsilon in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 epsilon antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of 14-3-3 epsilon in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 epsilon antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane1.: HeLa cell lysate
Lane2.: Jurkat cell lysate
Lane3.: 293T cell lysate
Lane4.: A549 cell lysate
Lane5.: Mouse Brain Tissue lysate

The Recombinant Protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane1.: 14-3-3 Zeta Recombinant Protein
Lane2.: 14-3-3 Beta Recombinant Protein
Lane3.: 14-3-3 Epsilon Recombinant Protein
Lane4.: 14-3-3 Eta Recombinant Protein
Lane5.: 14-3-3 Gamma Recombinant Protein
Lane6.: 14-3-3 Sigma Recombinant Protein
Lane7.: 14-3-3 Tau Recombinant Protein