Nkmaxbio/Human Thioredoxin Reductase 1/TRXR1 antibody/100ul/ATGA0451-100ul

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT30F1

UniProt No.

Q16881

NCBI Accession No.

NP_001087240

Alternative names

GRIM 12, KDRF, KM 102 derived reductase like factor, MGC9145, Oxidoreductase, Thioredoxin reductase, Thioredoxin reductase 1, Thioredoxin reductase 1 cytoplasmic, Thioredoxin reductase 1 Gene associated with retinoid IFN induced mortality 12 protein, Thioredoxin reductase GRIM 12, TR, TR 1, TRXR 1, TXNR, TXNRD 1

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human TXNRD1 (161-647aa) purified from E. coli

Isotype

IgG2b kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

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ICC/IF analysis of TXNRD1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXNRD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

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ICC/IF analysis of TXNRD1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXNRD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

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Western blot analysis (WB)

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TXNRD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane1.: HeLa cell lysate
Lane2.: A549 cell lysate
Lane3.: PC3 cell lysate