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LifeSensors/UM413, K63 Linkage Specific UbiTest K63 Linkage Specific UbiTest -TUBE Elution Kit/UM413, K63 Linkage Specific Ub

ThiskitallowsyoutodetectwhetheryourtargetproteinismodifiedbyK63poly-ubiquitin.

Determiningthelinkageofpolyubiquitinontargetproteinsischallenging.ThetrADItionalmethodsareeitherthroughMassSpectrometryorimmunoblotwithlinkagespecificantibodies,whicharecumbersome.LifeSensorshasdevelopedamoredefinitivemethodfordemonstratingtheubiquitylationlinkageofaprotein,whichistocoupleimmunoprecipitationofpolyubiquitylatedproteinwithdigestionbya linkagespecificdeubiquitylase priortoimmunoblotanalysis. AnincreasedsignalfortheunmodifiedsubstrateoradecreasedsignalofpolyubiquitylatedsubstrateathighmolecularweightafterK48/K63specificDUBtreatmentisaclearindicationthattheproteinwasK48/K63ubiquitylated. WehavebuiltthiskitaroundtheuseofTandemUbiquitinBindingEntities(TUBEs)whichbindtoallubiquitinchainlinkages.


ThiskitincludesthefollowingDUBs:
  • ALys63linkagespecificDUB-35µg,10µM
  • AbroadspectrumDUB-25µg,10µM 

ThisproductisprotectedbyoneormoreUSorForeignpatents.Pleasereadthe LimitedUseLabelLicense tolearnmore.Bypurchasingthisproduct,thepurchaseragreestocomplywith theseterms.


Info

KitContents:

Tube1Anti-Ub-TUBE1orTUBE2,Agarose–1mLofa50%slurry
Buffer:PBS,pH7.2,20%ethanol
Storage:-20°C.Avoidstorageatlowertemperature.Smallbatchesofequilibratedresincanbestoredat4°Cforupto1week

Tube2Elutionwashbuffer–2mL
Tube3Elutionbuffer–1mL
Tube4Neutralizationbuffer(10X)–0.5mL

Tube5USP2core–25ug
Storage:-80°C



Applications
  • Convenientone-steppull-downofpolyubiquitylatedproteinsfromcellandtissueextracts
  • Isolateubiquitylatedproteinsforproteomicstudies
  • Confirmationoftargetproteinubiquitylation

Benefits
  • Realproofofubiquitylation–nomassspecneeded!
  • Verificationthatthepatternobservedisfromubiquitin,nototherpost-translationalmodifications
  • Easycomparisonofpolyubiquitinlevelsbetweentreatedsamples
  • EasyscreeningformultiplePOIs
  • Nosubstratepurificationneeded
  • Controlforantibodynotrecognizingubiquitylatedsubstrate
  • Sufficientforaminimumof10pull-downs

Documents
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