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QED Bioscience/HIF-2α/34040/1 Ea


HIF-2αMonoclonalAntibody


ORDERINGINFORMATION

CatalogNo.:34040(cloneEP190b)Format:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.

BACKGROUND

Membersofthehypoxia-inducIBLefactor(HIF)familyoftranscriptionfactorsregulatecellularresponsestohypoxia.HIF-2αisinvolvedincatecholaminehomeostasis,vascularremodeling,physiologicalangiogenesis,andADIpogenesis.Itisoverexpressedinmanycanceroustissues.Studiessuggestthatinnon-smallcelllungcancer,HIF-2αisapromoteroftumorgrowthandprogressioninasolidtumor.

SPECIFICATIONSUMMARY

Antigen:Fusionproteincorrespondingtoaa535-631ofhumanHIF-2α.Accessionno.:Q99814,NP_001421.4HostSpecies:MouseAntibodyClass:IgG1Specificity:ThisantibodyrecognizeshumanHIF-2α.

APPLICATIONS

Immunoblotting:useat1ug/ml.Predictedmolecularweight~96kDa.

Immunofluorescence:useat10ug/ml.



DetectionofHIF-2αinCoCl-treatedHeLacelllysate.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DetectionofHIF-2αinformaldehyde-fixedHeLacells.


DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Storeinappropriatealiquotstoavoidmultiplefreeze-thawcycles.


Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.

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