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QED Bioscience/Recombinant CCL4 (MIP-1β)/50191P-1000/1 Ea


RecombinantCCL4(MIP-1β)



ORDERINGINFORMATIONORDERINGINFORMATION

CatalogNo.Size

50191P-22ug

50191P-1010ug

50191P-5050ug

50191P-100100ug

50191P-10001000ug


BACKGROUNDBACKGROUND

CCL4,alsoknownasMacrophageInflammatoryProtein-1beta(MIP-1β)isasmallcytokine(~9kDa)thatbelongstotheCCchemokinefamily.CCL4bindstoCCR1,CCR2isoformB,andCCR5.CCL4isoneofthemajorHIV-suppressivefactorsproducedbyCD8+T-cells;recombinantMIP-1βinducesadose-dependentinhibitionofdifferentstrainsofHIV-1,HIV-2,andsimianimmunodeficiencyvirus(SIV).TheprocessedformofMIP-1β(aa3-69)retainstheABIlitiestoinducedown-modulationofsurfaceexpressionofCCR5andtoinhibitCCR5-mediatedentryofHIV-1intoT-cells.


DESCRIPTIONDESCRIPTION

Source:RecombinanthumanCCL4isproducedinE.coli(accessionno.

P13236,aa24-92).

ProteinSequence:APMGSDPPTACCFSYTARKLPRNFVVDYYETSSLCSQPAVVFQTKRSKQVCADPSESWVQEYVYDLELN

MolecularMass:7.818kDabyMassSpec.

Purity:>97%bySDS-PAGE

Activity:EC50=0.45-0.63nMdeterminedbyMigrationAssayincellsexpressingrecombinantCCR5.

EndotoxinLevel:<0.01EUper1ugofproteinbyLALmethod.

Form:Lyophilized.

CarrierProtein:None.


PREPARATIONANDSTORAGEPREPARATIONANDSTORAGE

Reconstitution:Recommendedat100ug/mlinsteriledistilledwater.

StabilityandStorage:12monthsfromdateofreceipt,-20oCto-70oC,assupplied.

1month,-20oCto-70oC,understerileconditionsafterreconstitution.Bestifusedimmediatelyafterreconstitution.

Avoidmultiplefreeze-thawcycles.


Forinvitroinvestigationaluseonly.Notforuseindiagnosticortherapeuticprocedures.

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