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QED Bioscience/NIK (CT)/1129/1 Ea


Anti-NIK(CT)Antibody


ORDERINGINFORMATIONCatalogNo.:1129Size:100ugIgGinPBS,1mg/ml,pH7.4,0.02%sodiumazide.Purifiedbyimmuno-affinitychromatographyonthepeptideimmunogen.


BACKGROUND

NuclearfactorkappaB(NF-κB)isaubiquitoustranscriptionfactorandanessentialmediatorofgeneexpressionduringactivationofimmuneandinflammatoryresponses.NF-κBmediatestheexpressionofagreatvarietyofgenesinresponsetoextracellularstimuliincludingIL-1,TNFα,LPS,andmitogens.Aserine/threonineproteinkinasewhichmediatesNF-κBactivationbyIL-1,TNFα,andCD95hasbeenidentifiedanddesignatedNIK(forNF-κBinducingkinase).NIKisanactivatorofIκBkinasealphaandbeta(IKKαandIKKβ).Therefore,NIKisakeymoleculeintheNF-κBsignalingpathwayleADIngtotheexpressionofa

varietyofgeneproductsinresponseto

SPECIFICATIONSUMMARYAntigen:Syntheticpeptidecorrespondingtoaa931-947ofhumanNIK(accessionno.Q99558).HostSpecies:Rabbit


SPECIFICITY

ThisantibodyrecognizeshumanNIK.


APPLICATIONS

ELISA

Immunoblotting

Recommendedconcentration:2-4ug/ml.Optimaldilutionshouldbedeterminedbyenduser.

Positivecontrol:WholecelllysateofNIK-transfected293cells.


DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.


STORAGEANDSTABILITY

Thisantibodyisstablefor3monthsat

oo

pro-inflammatorycytokinesandbacterialproducts.

4Candatleast1yearat-20C.Avoid

multiplefreeze-thawcycles.


Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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