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Reachbio/Human Peripheral Blood CD4+ Helper T Cells/0504-100/15

ProductDescription

HumanPeripheralBloodCD4+HelperTCells

 

CellEnrichment

NegativeSelection

CD4+HelperTCellsareenrichedfromhumanperipheralblood(PB)mononuclearcells(MNCs)usinganegativeselectionimmunomagneticseparationmethodinvolvingofanantibodycocktailthattargetsanddepletesnon-CD4+cells.

 

SampleCollectionandProcessing

Peripheralbloodcellsarecollectedfromnormalhumanvolunteersvialeukapheresis(COBE®SpectraMNCcollectionprotocolorequivalent).Themononuclearcell-richleukocytelayer(leukapheresisproduct)iscontinuallyaspiratedbyalowflowratecollectionpumpdirectlyintoacollectionbag.ImmunomagneticcellenrichmentofCD4+cellsis performedwithin30hoursofcollection.DonorshavebeenscreenedandfoundtobenegativeforastandardpanelofinfectiousagentsthatatminimumincludesHIV,HBVandHCV.Dependingonthespecificlot,otherinfectiousagentsmayalsohavebeentestedfor(CertificateofAnalysisincludeslot-specifictestinginformation). 

Purity

PurityofCD4+HelperTCellsis>90%asmeasuredbyflowcytometricanalysis.

 

StABIlityandStorage

PeripheralbloodCD4+cellsarecryopreservedinCryoStor®CS5,aserum-freefreezingmedium.Cellsarestableforlong-termstorageat-152oCorinthegaseousphaseofliquidnitrogen.Storageat-80oCisnotrecommended.Oncethawed,thecellsmustbeusedimmediatelyandhaveafinitelifespaninculture.

 

IntendedUse

FORINVITRORESEARCHUSEONLY.NOTINTENDEDFORHUMANORVETERINARYUSEINVIVO.NOTTOBEUSEDFORANYDIAGNOSTIC,THERAPEUTICORCLINICALAPPLICATIONSWHATSOEVER. 

AdditionalInformation

CatalogNumberSelectionTypeUnitSize

(cellspervial)

Product

InfoSheet

0504-100Negative15milliondownload
Weight1lbs
Species

Human

TissueSource

PeripheralBlood

CellType

TCells,TCells–Helper

SelectionType

Negative

IdentifyingSurfaceMarker

CD4

UnitSize(millioncells/vial)

15

Format

Cryopreserved

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