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Reachbio/Human Peripheral Blood CD56+ Natural Killer (NK) Cells/0556-100/5 million

ProductDescription

HumanPeripheralBloodCD56+NaturalKiller(NK)Cells

 

CellEnrichment

NegativeSelection
NegativelyselectedCD56+NKCellsareenrichedfromhumanperipheralblood(PB)mononuclearcells(MNCs)usinganimmunomagneticcellseparationmethodthatinvolvesanantibodycocktailthattargetsanddepletesnon-CD56+cells. 
PositiveSelection
PositivelyselectedCD56+NKCellsarepurifiedfromhumanperipheralblood(PB)mononuclearcells(MNCs)usinganimmunomagneticcellseparationmethodthatinvolvesalabeledantibodythattargetstheCD56antigen.

 

SampleCollectionandProcessing

Peripheralbloodcellsarecollectedfromnormalhumanvolunteersvialeukapheresis(COBE®SpectraMNCcollectionprotocolorequivalent).Themononuclearcell-richleukocytelayer(leukapheresisproduct)iscontinuallyaspiratedbyalowflowratecollectionpumpdirectlyintoacollectionbag.ImmunomagneticcellenrichmentofCD56+cellsisperformedwithin30hoursofcollection.DonorshavebeenscreenedandfoundtobenegativeforastandardpanelofinfectiousagentsthatatminimumincludesHIV,HBVandHCV.Dependingonthespecificlot,otherinfectiousagentsmayalsohavebeentestedfor(CertificateofAnalysisincludeslot-specifictestinginformation).

 

Purity

PurityofCD56+NKCellsis>90%asmeasuredbyflowcytometricanalysis.

 

StABIlityandStorage

PeripheralbloodCD56+NKCellsarecryopreservedinCryoStor®CS5,aserum-freefreezingmedium.Cellsarestableforlong-termstorageat-152oCorinthegaseousphaseofliquidnitrogen.Storageat-80oCisnotrecommended.Oncethawed,thecellsmustbeusedimmediatelyandhaveafinitelifespaninculture.

 

IntendedUse

FORINVITRORESEARCHUSEONLY.NOTINTENDEDFORHUMANORVETERINARYUSEINVIVO.NOTTOBEUSEDFORANYDIAGNOSTIC,THERAPEUTICORCLINICALAPPLICATIONSWHATSOEVER. 

AdditionalInformation

CatalogNumberSelectionTypeUnitSize

(cellspervial)

Product

InfoSheet

0556-100Negative5milliondownload
0556-101Positive5milliondownload
Weight1lbs
Species

Human

TissueSource

PeripheralBlood

CellType

NKCells

SelectionType

Negative,Positive

IdentifyingSurfaceMarker

CD56

UnitSize(millioncells/vial)

5million

Format

Cryopreserved

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