This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant Ephrin-A3 extracellular domain.
Antigen:
recombinant human Ephrin-A3 EC domain
Stability:
Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
Reconstitution:
Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Description:
EphrinA3, also known as Ehk1L, EFL2, and LERK3, is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The Aclass ligands have a GPI anchor following the conserved sequence. EphrinA3 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, and EphB1. The extracellular domains of human and mouse EphrinA3 share 96% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation. In vivo, the ligands and receptors display reciprocal expression. It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue. The Eph/ephrin families also appear to play a role in angiogenesis.
