| AntibodyDilution | IHC(1:1000);optimaldilutionsforassaysshouldbedeterminedbytheuser. |
| Conjugates | AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated
| PerCP | Overview:- Peridinin-Chlorophyll-ProteinComplex
- Smallphycobiliprotein
- Isolatedfromredalgae
- Largestokesshift(195nm)
- MolecularWeight:35kDa
PerCPDatasheet |  | OpticalProperties: λex =482nm λem=677nm εmax=1.96x106 Laser =488nm | PE/ATTO594 | | PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm. | Overview:- Highfluorescenceyield
- HighphotostABIlity
- Veryhydrophilic
- Excellentsolubilityinwater
- Verylittleaggregation
PE/ATTO594Datasheet |  | OpticalProperties: λex =535nm λem =627 nm Laser = 488to561nm | FITC(Fluorescein) | Overview:- Excellentfluorescencequantumyield
- Highrateofphotobleaching
- Goodsolubilityinwater
- Broademissionspectrum
- pHdependentspectra
- Molecularformula:C20H12O5
- Molarmass:332.3g/mol
FITC-Fluorescent-conjugate |  | OpticalProperties: λex =494 nm λem=520 nm εmax=7.3×104 Φf= 0.92 τfl =5.0ns Brightness= 67.2 Laser = 488 nm Filterset = FITC | ATTO700 | Overview:- Highfluorescenceyield
- Excellentthermalandphotostability
- Quenchedbyelectrondonors
- Veryhydrophilic
- Goodsolubilityinpolarsolvents
- Zwitterionicdye
- MolarMass:575g/mol
ATTO700Datasheet |  | OpticalProperties: λex =700 nm λem=719nm εmax=1.25×105 Φf= 0.25 τfl =1.6ns Brightness= 31.3 Laser =676nm Filterset =Cy®5.5 | ATTO680 | Overview:- Highfluorescenceyield
- Excellentthermalandphotostability
- Quenchedbyelectrondonors
- Veryhydrophilic
- Goodsolubilityinpolarsolvents
- Zwitterionicdye
- MolarMass:631g/mol
ATTO680Datasheet |  | OpticalProperties: λex =680nm λem=700 nm εmax=1.25×105 Φf= 0.30 τfl =1.7ns Brightness=37.5 Laser =633 –676nm Filterset =Cy®5.5 | ATTO655 | Overview:- Highfluorescenceyield
- Highthermalandphotostability
- Excellentozoneresistance
- Quenchedbyelectrondonors
- Veryhydrophilic
- Goodsolubilityinpolarsolvents
- Zwitterionicdye
- MolarMass:634g/mol
ATTO655Datasheet |  | OpticalProperties: λex =663nm λem=684nm εmax=1.25×105 Φf= 0.30 τfl =1.8ns Brightness= 37.5 Laser =633 –647nm Filterset =Cy®5 | ATTO633 | Overview:- Highfluorescenceyield
- Highthermalandphotostability
- Moderatelyhydrophilic
- Goodsolubilityinpolarsolvents
- StableatpH4–11
- Cationicdye,perchloratesalt
- MolarMass:652.2g/mol
ATTO633Datasheet |  | OpticalProperties: λex =629nm λem=657nm εmax=1.3×105 Φf= 0.64 τfl =3.2ns Brightness=83.2 Laser =633 nm Filterset =Cy®5 | ATTO594 | Overview:- Highfluorescenceyield
- Highphotostability
- Veryhydrophilic
- Excellentsolubilityinwater
- Verylittleaggregation
- Newdyewithnetchargeof-1
- MolarMass:1137g/mol
ATTO594Datasheet |  | OpticalProperties: λex =601 nm λem=627nm εmax=1.2×105 Φf= 0.85 τfl =3.5ns Brightness=102 Laser =594nm Filterset =TexasRed® | ATTO565 | Overview:- Highfluorescenceyield
- Highthermalandphotostability
- Goodsolubilityinpolarsolvents
- Excellentsolubilityinwater
- Verylittleaggregation
- Rhodaminedyederivative
- MolarMass:611g/mol
ATTO565Datasheet |  | OpticalProperties: λex =563nm λem=592nm εmax=1.2×105 Φf= 0.9 τfl =3.4n Brightness=10 Laser =532nm Filterset = TRITC | ATTO488 | Overview:- Highfluorescenceyield
- Highphotostability
- Veryhydrophilic
- Excellentsolubilityinwater
- Verylittleaggregation
- Newdyewithnetchargeof-1
- MolarMass:804g/mol
ATTO488Datasheet |  | OpticalProperties: λex =501nm λem=523nm εmax=9.0×104 Φf= 0.80 τfl =4.1ns Brightness= 72 Laser = 488 nm Filterset = FITC | ATTO390 | Overview:- Highfluorescenceyield
- LargeStokes-shift(89nm)
- Goodphotostability
- Moderatelyhydrophilic
- Goodsolubilityinpolarsolvents
- Coumarinderivate,uncharged
- Lowmolarmass:343.42g/mol
ATTO390Datasheet |  | OpticalProperties: λex =390nm λem=479nm εmax=2.4×104 Φf= 0.90 τfl =5.0ns Brightness= 21.6 Laser =365or405nm | APC(Allophycocyanin) | Overview:- Highquantumyield
- Largephycobiliprotein
- 6chromophorespermolecule
- Isolatedfromredalgae
- MolecularWeight:105kDa
APCDatasheet |  | OpticalProperties: λex =650nm λem=660nm εmax=7.0×105 Φf= 0.68 Brightness= 476 Laser =594or633 nm Filterset =Cy®5 StreptavidinProperties: - Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinity
- Molecularweight:53kDa
- Formula:C10H16N2O3S
- Applications:Westernblot,immunohistochemistry,andELISA
StreptavidinDatasheet Biotin Properties: - BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinity
- Molarmass:244.31g/mol
- Formula:C10H16N2O3S
- Applications:Westernblot,immunohistochemistry,andELISA
BiotinDatasheet HRP(HorserADIshperoxidase)Properties: - Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
- Readilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonors
- Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
- Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
- Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
- Highturnoverrateenablesrapidgenerationofastrongsignal
- 44kDaglycoprotein
- Extinctioncoefficient:100(403nm)
- Applications:Westernblot,immunohistochemistry,andELISA
HRPDatasheet AP(AlkalinePhosphatase)Properties: - Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenols
- Commonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligation
- Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproducts
- Fluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproducts
- Molecularweight:140kDa
- Applications:Westernblot,immunohistochemistry,andELISA
APDatasheet | R-PE(R-Phycoerythrin) | Overview:- Broadexcitationspectrum
- Highquantumyield
- Photostable
- Memberofthephycobiliproteinfamily
- Isolatedfromredalgae
- Excellentsolubilityinwater
- MolecularWeight:250kDa
R-PEDatasheet |  | OpticalProperties: λex =565nm λem=575nm εmax=2.0×106 Φf= 0.84 Brightness=1.68x103 Laser = 488to561nm Filterset = TRITC ProductImages ImmunohistochemistryanalysisusingMouseAnti-DNADamageMonoclonalAntibody,Clone15A3(SMC-155).Tissue:RetinalInjuryModel.Species:Mouse.PrimaryAntibody:MouseAnti-DNADamageMonoclonalAntibody(SMC-155)at1:1000.SecondaryAntibody:AlexaFluor594GoatAnti-Mouse(red).Courtesyof:Dr.RajashekharGangaraju,UniversityofIndiana,DepartmentofOpthamology,EugeneandMarilynGlickEyeInstitute.  ImmunohistochemistryanalysisusingMouseAnti-DNADamageMonoclonalAntibody,Clone15A3(SMC-155).Tissue:inflamedcolon.Species:Mouse.Fixation:Formalin.PrimaryAntibody:MouseAnti-DNADamageMonoclonalAntibody(SMC-155)at1:1000000for12hoursat4°C.SecondaryAntibody:BiotinGoatAnti-Mouseat1:2000for1houratRT.Counterstain:MayerHematoxylin(purple/blue)nuclearstainat200µlfor2minutesatRT.Magnification:40x.  ImmunohistochemistryanalysisusingMouseAnti-DNADamageMonoclonalAntibody,Clone15A3(SMC-155).Tissue:backskin.Species:Mouse.Fixation:Bouin’sFixativeandparaffin-embedded.PrimaryAntibody:MouseAnti-DNADamageMonoclonalAntibody(SMC-155)at1:100for1houratRT.SecondaryAntibody:FITCGoatAnti-Mouse(green)at1:50for1houratRT.  ImmunohistochemistryanalysisusingMouseAnti-DNADamageMonoclonalAntibody,Clone15A3(SMC-155).Tissue:Ischemicfreshbraintissue.Species:Rat.PrimaryAntibody:MouseAnti-DNADamageMonoclonalAntibody(SMC-155)at1:1000for16hoursatRT.SecondaryAntibody:AlexaFluor546GoatAnti-mouse(Red)at1:500for1houratRT.Localization:CerebralCortex.Courtesyof:Dr.YiYang,U.NewMexico. ProductCitations(10)ImmunohistochemistryLossofangiotensinIIreceptorexpressionindopamineneuronsinParkinson’sdiseasecorrelateswithpathologicalprogressionandisaccompaniedbyincreasesinNox4-and8-OHguanosine-relatednucleicacidoxidationandcaspase-3activation. Zawada,W.M.etal.(2015)ActaNeuropatholCommun.3(1):9. PubMedID:25645462ReactivityHumanApplications:Immunohistochemistry Epilepsy:neuroinflammation,neurodegeneration,andAPOEgenotype. Aboud,O.,MrakR.E.,BoopF.A.,andGriffinW.S.T.(2013)ActaNeuropatholCommun.1(1):41. PubMedID:24252240ReactivityHumanApplications:Immunohistochemistry Immunocytochemistry/Immunofluorescence17β-Estradiolenhancessulforaphanecardioprotectionagainstoxidativestress. Angeloni,C.etal.(2017)JNutriBiochem.42(2017)26-36. PubMedID:N/AReactivityRatApplications:Immunocytochemistry/Immunofluorescence Invivooxidativestressaltersthiolredoxstatusofperoxiredoxin1and6andimpairsratspermquality. Liu,Y.andO"Flaherty,C.(2016)AsianJAndrol.19(1):73-79. PubMedID:26823067ReactivityRatApplications:Immunocytochemistry/Immunofluorescence NeutrophilextracellulartrapsenrichedinoxidizedmitochondrialDNAareinterferogenicandcontributetolupus-likedisease. Lood,C.etal.(2016)NatMed.22(2):146-53. PubMedID:26779811ReactivityVariousspeciesApplications:Immunocytochemistry/Immunofluorescence NeutrophilextracellulartrapsenrichedinoxidizedmitochondrialDNAareinterferogenicandcontributetolupus-likedisease. Lood,C.etal.(2016)NatMed.22(2):146-53. PubMedID:26779811ReactivityVariousspeciesApplications:Immunocytochemistry/Immunofluorescence Neuroprotectiveeffectofsulforaphaneagainstmethylglyoxalcytotoxicity. Angeloni,C.etal.(2015)ChemResToxicol.28(6):1234-45. PubMedID:25933243ReactivityHumanApplications:Immunocytochemistry/Immunofluorescence Advancingageincreasesspermchromatindamageandimpairsfertilityinperoxiredoxin6nullmice. Ozkosem,B.,Feinstein,S.I.,Fisher,A.B.andO"Flaherty,C.(2015)RedoxBiol.5:15-23. PubMedID:25796034ReactivityMouseApplications:Immunocytochemistry/Immunofluorescence AbsenceofPEROXIREDOXIN6isAssociatedwithLowSpermChromatinQualityandSubfertilityinMiceChallengedwithOxidativeStress. Ozkosem,B.andO"Flaherty,C.(2013)figshare. PubMedID:N/AReactivityMouseApplications:Immunocytochemistry/Immunofluorescence OtherCitationsDisruptionofredoxhomeostasisleadstooxidativeDNAdamageinspermatocytesofWolbachia-infectedDrosophilasimulans. Brennan,L.J.,Haukedal,J.A.,Earle,J.C.,Keddie,B.andHarris,H.L.(2012)InsectMolBiol.21(5):510-520. PubMedID:22831171ReactivityAedesalbopictusApplications:DotBlot | ATTO390 | Overview:- Highfluorescenceyield
- LargeStokes-shift(89nm)
- Goodphotostability
- Moderatelyhydrophilic
- Goodsolubilityinpolarsolvents
- Coumarinderivate,uncharged
- Lowmolarmass:343.42g/mol
ATTO390Datasheet | | OpticalProperties: λex =390nm λem=479nm εmax=2.4×104 Φf= 0.90 τfl =5.0ns Brightness= 21.6 Laser =365or405nm
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