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TheOKT8antibodyisspecificforthe32-34kDaalphachainofhumanCD8,knownasCD8aorCD8alpha.CD8acanformahomodimer(CD8alpha-alpha),butismorecommonlyexpressedasaheterodimerwithasecondchainknownasCD8borCD8beta.CD8actsasaco-receptorforantigenrecognitionandsubsequentTcellactivationthatisinitiateduponbindingoftheTcellreceptor(TCR)toantigen-bearingMHCClassImolecules.ThecytoplasmicdomainsofCD8providebindingsitesforthetyrosinekinaselck,facilitatingintracellularsignalingeventsthatleadtoTcellactivation,development,andcytotoxiceffectorfunctions.CD8+cytotoxicTcells(CTLs)playanimportantroleininducingcelldeathoftumorcells,aswellascellsinfectedbyvirus,bacteriaorparasites.
TheOKT8antibodyiswidelyusedasaphenotypicMarkerforCD8oncytotoxicTcells,thymocytes,aswellasoncertaincelltypesthatdonotalsoexpresstheTCR,includingsomeNKcellsandlymphoiddendriticcells.IfusedtogetherwithalternativeantibodiesAnti-HumanCD8acloneRPA-T8orAnti-HumanCD8acloneHit8a,theOKT8antibodywillnotblockbindingofRPA-T8orHit8a.
| Name | PEAnti-HumanCD8a(OKT8) |
|---|---|
| Cat.No. | 50-0086 |
| AlternativeNames | CD8alpha,leu-2a |
| GeneID | 925 |
| Clone | OKT8 |
| Isotype | MouseIgG2a |
| Reactivity | Human |
| CrossReactivity | |
| Format | PE |
| Applications | FlowCytometry |
| Citations* | JahnkeM,TrowsdaleJ,andKellyAP.2012.J.Biol.Chem.287:28779-28789.(FlowCytometry,Immunoprecipitation) ClementM,LadellK,Ekeruche-MakindeJ,MilesJJ,EdwardsESJ,DoltonG,WilliamsT,SchauenburgAJA,ColeDK,LauderSN,GallimoreAM,GodkinAJ,BurrowsSR,PriceDA,SewellAK,andWooldridgeL.2011.J.Immunol.187:654-663.(invitroactivation) BagnaraD,KaufmanMS,CalissanoC,MarsilioS,PattenPEM,SimoneR,ChumP,YanX-Y,AllenSL,KolitzJE,BaskarS,RadarC,MellstedtH,RabbaniH,LeeA,GregersenPK,RaiKR,andChiorazziN.2011.Blood.117:5463-5472.(invivoactivation) TelesRMB,KrutzikSR,OchoaMT,OliveiraRB,SarnoEN,andModlinRL.2010.78:4634-4643.(Immunohistochemistry–OCTembeddedfrozentissue) LaiAY,FatemiM,DhasarathyA,MaloneC,SobolSE,GeigermanC,JayeDL,MavD,ShahR,LiL,andWadePA.2010.J.Exp.Med.207:1939-1950.(invitroTcelldepletion) ThakralD,DobbinsJ,DevineL,andKavathasPB.2008.J.Immunol.180:7431-7442.(Immunoprecipitation) VargheseJCandKaneKP.2008.J.Immunol.181:6002-6009.(invitroblocking) |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn