The145-2C11antibodyisspecificformouseCD3e,alsoknownasCD3epsilon,a20kDasubunitoftheTcellreceptorcomplex,alongwithCD3gammaandCD3delta.TheseintegralmembraneproteinchainsassemblewithadditionalchainsoftheTcellreceptor(TCR),aswellasCD3zetachain,toformtheTcellreceptor–CD3complex.Togetherwithco-receptorsCD4orCD8,thecomplexservestorecognizeantigensboundtoMHCmoleculesonantigen-presentingcells.SuchinteractionspromoteTcellreceptorsignaling(Tcellactivation)andcanresultinanumberofcellularresponsesincludingproliferation,differentiation,productionofcytokinesoractivation-inducedcelldeath.CD3isdifferentiallyexpressedduringthymocyte-to-TcelldevelopmentandonallmatureTcells.
The145-2C11antibodyisawidelyusedphenotypicMarkerformouseTcells.Inaddition,bindingof145-2C11antibodytoCD3ecaninducecellactivation.ArecentpublicationofthecrystalstructureofamurineCD3e-mitogenicantibodycomplexprovidesfurtherinsightintotheactionofcommonlyusedagoNISTantibodies(Fernandes,R.A.etal.2012.J.Biol.Chem.287:13324-13335).
Name | PurifiedAnti-MouseCD3e(145-2C11) |
---|---|
Cat.No. | 70-0031 |
AlternativeNames | CD3epsilon |
GeneID | 12501 |
Clone | 145-2C11 |
Isotype | ArmenianHamsterIgG |
Reactivity | Mouse |
CrossReactivity | |
Format | Purified |
Applications | FlowCytometry,IF,IHCF,IP,WesternBlot |
Citations* | StaehliR,LudigsK,HeinzLX,Sequin-EstevezQ,FereroI,BraunM,SchroderK,RebsamenM,TardivelA,MattmannC,MacDonaldHR,RomeroP,ReithW,GuardaG,andTschoppJ.2012.J.Immunol.188:3820-3828.(invitroactivation) TodoT,WuG,ChaiNN,HeY,MartinsG,GuptaA,FairJ,LiuNY,JordanS,andKleinA.2012.Int.Immunol.10:1093.(invivoassay) MiraE,LeonB,BarberDF,Jimenez-BarandaS,GoyaI,AlmonacidL,MarquezG,ZaballosA,MartinezC,SteinJV,ArdavinCandManesS.2012.J.Immunol.181(5):3524-3534.(invitroactivation,Immunohistochemistry–frozentissue) Becker-HermanA,Meyer-BahlburgA,SchwartzMA,JacksonSW,HudkinsKL,LiuC,SatherBD,KhimS,LiggittD,SongW,SilvermanGJ,AlpersCEandRawlingsDJ.2011.J.Exp.Med.208:2033-2042.(Immunofluorescencemicroscopy–OCTembeddedfrozentissue) SalmondRJ,FilbyA,PirinenN,MageeAI,andZamoyskaR.2010.Blood.117:108-117.(Immunoprecipitation) TilleySL,JaradatM,StapletonC,DixonD,HuaX,EriksonCJ,.McCaskillJG,ChasonKD,LiaoG,JaniaL,KollerBH,andJettenAM.2007.J.Immunol.178:3208-18.(Immunohistochemistry–frozentissue) IsakovN,WangeRL,BurgessWH,WattsJD,AebersoldR,andSamelsonLE.1995.J.Exp.Med.181:375-380.(invitroactivation,Immunoprecipitation) SalvadoriS,GansbacherB,PizzimentiAM,andZierKS.1994.J.Immunol.153:5176-5182.(WesternBlotting) LeoO,FooM,SachsD,SamuelsonL,andBluestoneJ.1987.Proc.Natl.Acad.Sci.USA.84:1374(Originationofclone145-2C11,invitroactivation,invitroblocking,Immunoprecipitation) |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn