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TheGK1.5antibodyreactswithmouseCD4,a55kDaproteinwhichactsasaco-receptorfortheTcellreceptor(TCR)initsinteractionwithMHCClassIImoleculesonantigen-presentingcells.TheextracellulardomainofCD4bindstothebeta-2domainofMHCClassII,whileitscytoplasmictailprovidesabindingsiteforthetyrosinekinaselck,facilitatingthesignalingcascadethatinitiatesTcellactivation.CD4istypicallyexpressedonthymocytes,certainmatureTcellpopulationssuchasTh17andTregulatory(Treg)cells,aswellasondendriticcells.
TheGK1.5antibodyiswidelyusedasaphenotypicMarkerforCD4expression.Ifusedtogether,theGK1.5antibodyandanalternativeantibody,Anti-MouseCD4cloneRM4-5,will“compete”forbinding,i.e.RM4-5isabletoblockGK1.5bindingtocells.Incontrast,theAnti-MouseCD4cloneRM4-4doesnotblockbindingoftheGK1.5antibodytocells(AroraSetal.2006.Infect.Immun.74:4339-4348).TheGK1.5antibodyisalsoreportedtobecross-reactivewithSyrianhamsterCD4.
| Name | PE-Cyanine7Anti-MouseCD4(GK1.5) |
|---|---|
| Cat.No. | 60-0041 |
| AlternativeNames | L3T4,T4 |
| GeneID | 12504 |
| Clone | GK1.5 |
| Isotype | RatIgG2b,kappa |
| Reactivity | Mouse |
| CrossReactivity | SyrianHamster |
| Format | PE-Cyanine7 |
| Applications | FlowCytometry |
| Citations* | TaniguchiRT,DeVossJJ,MoonJJ,SidneyJ,SetteA,JenkinsMK,andAndersonMS.2012.Proc.Natl.Acad.Sci.109:7847-7852.(invivodepletion) WolkersMC,Gerlach,C,ArensR,JanssenEM,FitzgeraldP,SchumacherTN,MedemaJP,GreenDR,andSchoenbergerSP.2012.Blood.119:798-804.(invivodepletion) LeeL-F,LogronioK,TuGH,ZhaiW,NiI,MeiL,DilleyJ,YuJ,etal.2012.Proc.Natl.Acad.Sci.10.1073.(Flowcytometry). ThorntonEE,LooneyMR,BoseO,SenD,SheppardD,LocksleyR,HuangX,andKrummelMF.2012.J.Exp.Med.10:1084.(Immunohistochemistry–OCTembeddedfrozentissue) StephenTL,WilsonBS,andLauferTM.2012.Proc.Natl.Acad.Sci.109:7415-7420.(Immunoprecipitation) HammerbeckCDandHooperJW.2011.J.Virol.85(19)9929-9944.(Flowcytometry–Syrianhamster). DiamondMS,KinderM,MatsushitaH,MashayekhiM,DunnGP,ArchambaultJM,LeeH,ArthurCD,WhiteJM,KalinkeU,MurphyKM,andSchreiberRD.2011.J.Exp.Med.208:1989-2003.(invitroblocking) KaoH,LinJ,LittmanDR,ShawAS,andAllenPM.2008.J.Immunol.181:8248-8257.(Immunoprecipitation) FelixNJ,DonermeyerDL,HorvathS,WaltersJJ,GrossM,suriA,andAllenPM.2007.Nat.Immunol.8:388-397.(invitroblocking) |
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