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Adipogen/Ubiquitin vinyl methyl ester (human) (rec.)/SBB-PS0033-C050/50 µg

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ProductDetails
ProductTypeProtein
Properties
Source/HostE.coli
SequenceHumanubiquitin(aa1-76)(AccessionNr.P0CG47)withaC-terminalvinylmethylestergroup.
CrossreactivityHuman
ApplicationTypicalworkingconcentrationrangeis1-5μM.Reactionconditionswillneedtobeoptimizedforeachspecificapplication.
MW~9kDa
Purity≥97%(LCMS)
ConcentrationLotdependent.
AccessionNumberP0CG47
FormulationLiquid.In50mMMESpH6.0,100mMsodiumchloride.
OtherProductDataClickhereforaTypicalLot-specificProductDatasheetfromtheOriginalManufacturer
Ourproductdescriptionmaydifferslightlyfromtheoriginalmanufacturersproductdatasheet.
DeclarationManufacturedbySouthBayBio.
ShippingandHandling
ShippingDRYICE
ShortTermStorage-20°C
LongTermStorage-80°C
HandlingAdviceAliquottoavoidfreeze/thawcycles.
Use/StABIlityStableforatleast1yearafterreceiptwhenstoredat-80°C.
Documents
MSDSNo
ProductSpecificationSheet
DatasheetDownloadPDF
Ubiquitinisasmall(8.5kDa)regulatoryproteinthathasbeenfoundinalmostalltissuesofeukaryoticorganisms.Theadditionofubiquitintoasubstrateproteiniscalledubiquitinationorubiquitylation.Ubiquitinationcanaffectproteinsinmanyways:itcansignalfortheirdegradationviatheproteasome,altertheircellularlocation,affecttheiractivityandpromoteorpreventproteininteractions.Removalofubiquitinfromasubstrateproteinoccursviadeconjugatingenzymes,ofwhichtherearenearly100knownenzymeswithvariouslinkagespecificities.Thisproductconsistsofafull-lengthhuman,matureubiquitinpolypeptide(aminoacids1-76),expressedinE.coliwithaC-terminalwarhead(VinylMethylEster).Ubiquitinvinylmethylesterisapotent,irreversIBLeandspecificinhibitorofdeubiquitinatingenzymes(DUBs)basedonaC-terminalelectrophilicvinylmethylestergroup.UbiquitinvinylmethylestercanbeusedforactivityprofilingexperimentsanddeterminingDUBinhibitorspecificity.IttargetsthreeofthefourmajorDUBfamilies:UCH(UbiquitinC-terminalhydrolases),USP(Ubiquitinspecificproteases),OTU(Ovariantumorproteases)andMJD(Machado-Josephindomainproteases)whileJAMMmetalloproteasesarenotinhibited.
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