ProZyme/Glyko® Endo F2 [GKE-5008]/GKE-5008/6 mU

Description

Cleaveswithinthechitobiose(GlcNAc-GlcNAc)coreofhighmannoseandbiantennarycomplextypeN-glycans. EndoglycosidaseF2, endo-beta-N-acetylglucosaminidase. 

Source:
RecombinantgenefromElizabethkingiameningosepticum,expressedinE.coli.Thesourceorganismwaspreviouslyknownas Chryseobacterium[Flavobacterium]meningosepticum^1. 

Specificity:
Theenzymecleaveswithinthedi-N-acetylchitobiose (GlcNAc-GlcNAc)coreofhighmannoseandbiantennarycomplextypeN-linkedglycansofoligosaccharide,glycopeptideorglycoproteinsubstrates.AsingleGlcNAcresidueremainsattachedtothemoleculeafterhydrolysis,therestoftheglycanisreleased.ThisspecificityisdistinctfromPNGaseF(GKE-5006),whichcleavesbetweenasparagineandtheinnermostGlcNAcofN-linkedglycans.EndoF2cleavesbetweenGlcNAc-GlcNacin biantennaryglycansatarateapproximately20timeshigherthanhighmannoseglycans.Theenzymeisnotactivetowardshybridtypeortri-andtetraantennaryoligosaccharides.

Figure1:CleavagespecificityforGKE-5008 EndoF2.

R₁:N&CsubstitutionotherthanH
R₂: maybeHortherestofanoligosaccharide
R₃: maybeHor α(1-6)-linkedfucose

Applications:
SelectivereleaseofhighmannoseandbiantennarycomplextypeN-linkedoligosaccharidesfromglycopeptides/glycoproteins.

Shipswith:
WS01615XReactionBuffer,whichwhendilutedgives200mMsodiumacetatepH4.5.

Formulation:
10mMsodiumacetatepH4.5,25mMNaCl,0.02%sodiumazide

pHoptimum:
4.5

UnitDefinition:
Onemilliunitisdefinedastheamountofenzymethatwillhydrolyze1nmoleofthedansylatedporcinefibrinogenglycopeptidesubstrateDnsVGEN(CHO)RperminuteatpH4.5and37°C.

Size: 6mU(60µl)

Concentration: ≥100mU/ml

ProductCode: GKX-5008

1.KimKK,KimMK,LimJH,ParkHY,LeeST. TransferofChryseobacteriummeningosepticumandChryseobacteriummiricolatoElizabethkingiagen.nov.asElizabethkingiameningosepticacomb.nov.andElizabethkingiamiricolacomb.nov. IntJSystEvolMicrobiol.2005May;55(Pt3):1287-93.