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DESCRIPTION:Human recombinant PB1-3 bromodomain (third bromodomain of polybromo-1). PB1, a chromatin targeting component of the PBAF (SWI/SNF-B) chromatin-remodeling complex, comprises six tandem bromodomains. Mutations in PB1, including PB1-3, occur frequently in renal cancers. PB1-3 shows binding affinity for various acetyllysines in singly acetylated histone peptides (e.g. H2AK15Ac).
ACCESSION #: NM_018313
Uniprot Link
INCLUDES AMINO ACIDS: 374-494
TAG(S): N-terminal His-tag
MW: 17.3 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris/HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v)
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.
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