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Reaction Biology/SIRT3/KDA-11-423

DESCRIPTION:HumanrecombinantSIRT3.Amemberofthe“sirtuins”orclassIII(NAD-dependent)histonedeacetylases(HDACs)whichcatalyzesthedeacetylationofproteinacetyllysineresiduesinareactionthatformsnicotinamideandO-acetyl-ADP-ribosefromtheco-substrateNADandthelysine’sacetylfunction.

ACCESSION#:NM_012239.3

UniprotLink

INCLUDESAMINOACIDS:101-399

TAG(S):N-terminalGST-tag

MW:59.6kDa

EXPRESSIONSYSTEM:E.coli

SUPPLIEDAS:Solutionofpurifiedrecombinantproteinin50mMTris/HClpH7.5,500mMNaCl,10%glycerol(v/v).

STORAGE:-70°C.Thawquicklyandstoreonicebeforeuse.Theremaining,unused,undilutedenzymeshouldbesnapfrozen,forexampleinadry/iceethanolbathorliquidnitrogen.Minimizefreeze/thawsifpossIBLe,butverylowvolumealiquots(<5ul)orstorageofdilutedenzymeisnotrecommended.

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