Synonyms | MonoclonalMBPantibody,Anti-MBPantibody,MBPantibody,Maltosebindingproteinantibody,AntiMBPantibody,Maltosebindingproteinantibody |
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Specificity | Human |
CrossReactivity | Tobedeterminedbyend-user. |
Applications | ELISA,WB |
Immunogen | MBPantibodywasraisedinmouseusingrecombinantMBPpurifiedfromE.coliastheimmunogen. |
Host | Mouse |
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Clone | 10C12 |
Isotype | IgG1kappa |
Source | Anti-MBPantibody,clone10C12,isderivedfromhybridizationofmouseSP2/0myelomacellswithspleencellsfromBALB/cmiceimmunizedwitharecombinantMBPprotein. |
MethodofPurification | MBPantibodywaspurifiedbyprotein-Gaffinitychromatography |
Form&Buffer | SuppliedasaliquidinPBS,pH7.4,with0.1%NaN3. |
Concentration | 1mg/ml |
Storage | Storeat4degCforshorttermstorage.Aliquotandstoreat-20degCforlongtermstorage.Avoidrepeatedfreeze/thawcycles. |
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UsageRecommendations | WB:1:1,000-1:2,000 |
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BIOLOGicalSignificance | EpitopetaggingoffersaneasyanduniversalstrategyfortheidentificationandpurificationofproteinsderivedbyrecombinantDNAtechnology.TheinsertionofaMaltoseBindingProtein(MBP)tagcreatesastablefusionproductthatdoesnotinterferewiththebioactivityoftheproteinorwiththebiodistributionoftheMBPtaggedproduct. |
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