Synonyms | Monoclonal PNMT antibody, Anti-PNMT antibody, Phenylethanolamine-N-methyltransferase antibody, Monoclonal PNMT antibody, Anti-PNMT antibody, Phenylethanolamine-N-methyltransferase antibody |
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Cross Reactivity | Human |
Applications | FC, IF, WB |
Immunogen | PNMT antibody was raised in mouse using a full length recombinant protein of human PNMT (NP_002677) produced in HEK293T cells, as the immunogen. |
Host | Mouse |
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Clone | 1F3 |
Isotype | IgG1 |
Method of Purification | PNMT antibody was purified by affinity chromatography. |
Form & Buffer | Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
Concentration | 500 ug-1 mg/ml |
Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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Usage Recommendations | FC: 1:100, IF: 1:100, WB: 1:2000 |
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Biological Significance | Phenylethanolamine-N-methyltransferase (PNMT) is an enzyme converting noradrenaline to adrenaline. The enzyme is present in adrenomedullary cells and in the brain neurons. Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes the staining. |
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