Product Description
Size: 100 μl
Ab Type: Polyclonal
Reactivity: Eukaryotes
Immunogen: Di-methylated H3 (Lys79)-KLH
Host: Rabbit
Product DescriptionThis product is produced by immunizing rabbits with a synthetic di-methyl peptide corresponding to residues surrounding Lys79 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by di-methylated histone H3 (Lys79) peptide affinity chromatography.
SpecificityThis antibody detects histone H3 only when it is di-methylated at Lys79, but not the mono- or tri-methylated H3 peptide at Lys79 and the non-methylated peptide.
Recommended ApplicationsChIP, IP. Not tested in other applications*IP=Immunoprecipitation ChIP=Chromatin precipitation.
Recommended antibody dilutionIP: 1:100 ChIP: 1:100 (1 μl of antibody for 1x 105 HeLa)
ImportantUse at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.
A. Dot blotting analysis on indicated amount of di-methylated H3 peptide at Lys79 (lane 1), mono-methylated H3 peptide at Lys79 (lane 2), tri-methylated H3 peptide at Lys79 (lane 3) and non-methylated H3 peptide at Lys79 (lane 4) using di-methyl-histone H3 (Lys79) rabbit pAb.
B. Sonicated chromatin prepared from 1 x 105 HeLa cells was subjected to chromatin immunoprecipitation (ChIP) using either 1μl Di-methyl-Histone H3 (Lys79) rabbit pAb or 1 μg of normal rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human RPL30 Exon 3 and MyoD1 Exon 1. Data are presented as enrichment of each IPed sample relative to total amount of input chromatin at each amplicon.
Scientific DescriptionThe ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation in histones modulated by specific histone methylases (HMTs) and histone demethylases (HDMs) is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.
Storage & StabilityThe antibody is delivered in lyophilized powder. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds, and then reconstitute it with the antibody stabilizer provided. The stabilizer contains PBS, 50% glycerol, and 0.01% sodium azide. Please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Antibody is stable for 12 months from date of reconstitution. Equilibrate the antibody to room temperature for 2 minutes and mix by gently pipetting before use.**Research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
