产品资料

Ptmbiolabs/Histone H2A rabbit pAb/1/PTM-1008

Product Description

General Information
Catalog number	PTM-1008
Size	100 μl at 0.5 mg/ml
Clonality	Polyclonal
Species reactivity	Human, Monkey, Mouse, Rat
Host species	Rabbit
Immunogen	H2A-KLH
Form	Liquid
Product Information
Application	ELISA, Dot Blot, Western Blot, IHC
Dilution	Western Blot	1:2000
	IHC	1:500
Images
WB data
(A) (B)
Western blotting analysis on 30 μg of crude proteins from HeLa, COS-7, BRL whole cell lysates, and Liver, Kidney tissue lysate (Figure A) or 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4hr) (Figure B) using histone H2A rabbit pAb (1:2000).The membrane was re-probed by anti-acetyl-histone H2A (Lys5) rabbit pAb (1:2000).
IHC data
	Immunohistochemical analysis of paraffin-embedded human neuroblastoma tissue, labeling H2A with PTM-1008 at 1/500 dilution.Nuclear staining on tumor cells of human neuroblastoma was observed. Counter stained with hematoxylin.
Scientific Description
The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries. Histone H2A helps regulate chromatin structure and function through post-translational modifications and specialized histone variants. It is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.
Source/Purification
This product is produced by immunizing rabbits with a synthetic peptide corresponding to C terminus of human histone H2A. Antibodies are purified by peptide affinity chromatography.
Storage & Stability
The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.
References
1. Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 2009, 8(2):900-9062. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 2009, 8(1):45-52.3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819.**Research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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Ptmbiolabs/Histone H2A rabbit pAb/1/PTM-1008