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Ptmbiolabs/Pan anti-di-methyllysine antibody conjugated agarose beads/PTM-608

ProductDescriptionSize:0.5mLAbType:PolyclonalReactivity:All
ProductDescriptionAmixtureofhighqualityrabbit-derivedpolyclonalantibodiesiscross-linkedtoagarosebeadswithstableamidelinkages.Withthecombinedadvantagesofdifferentpolyclonalantibodiesinglobalproteomicscreeningofdi-methyllysine,thisproductpresentsextraordinarycapABIlitytospecificallycapturethepeptides/proteinsbearinglysinedi-methylatedresidues.Formulation0.25mLofdrainedagarosein0.5mLof50%glycerolslurry(with1mgofantibodyimmobilization).SpecificityWiththeimmobilizationofhighlyspecificanti-di-methyllysineantibody,thedi-methyllysineantibodyagarosebeadscapturepeptides/proteinsbearingdi-methyllysineresiduesbutdonotcross-reactwiththepeptides/proteinsbearingstructurallysimilarpropionyllysineorbutyryllysineresidues.ApplicationProtein/peptideimmunoprecipitationfordi-methyllysineproteomics;Immuno-affinitychromatography.
ScientificDescriptionThepost-translationalε-aminolysinemethylationofproteinsisanimportantreversIBLemodificationcontrollingproteinactivity.Thereversiblelysinemethylationofproteinsplaysavitalroleintheregulationofmanycellularprocessesincludingchromatinremodeling,geneexpression,cellproliferation,etc.Methylationoflysineresiduesismodulatedbyspecificcounteractiveenzymesincludinglysinemethylases(KMTs),demethylases(KDMs),histonemethylases(HMTs),andhistonedemethylases(HDMs),whosepathwayshavebecomepromisingtargetsfordiscoveryofanti-cancerdrugs.Storage&StabilityStoreproductat-20℃andavoidfreeze/thawcycles.Stablefor12monthsfromdateofreceipt.**Researchpurposesonly.Theproductisnotintendedtobeusedfortherapeuticordiagnosticpurposesinhumansoranimals.
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