ThiskitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"sCDNA.
Note:allgeneandpseudogenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
PseudogenesareDNAsegmentsintroducedintothegenomebygeneduplicationorRNAretrotransposition.Themajorityofpseudogenesbearhighsequencesimilaritytotheircorrespondingfunctionalgenes,buthavelostsomeorallofthefunctionality.Sciencell"sGeneQuery™HumanVIM-VIM2PPseudogeneTranscriptionAnalysisqPCRKit(GQP-VIM2P)isdesignedtofacilitatethetranscriptquantificationofpseudogeneVIM2Panditshighlyrelatedgene,VIM.HumanVIM2PpseudogeneandVIMgenearelocatedonchromosome6and10,respectively. GQP-VIM2Pguaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutnon-specificamplificationforprecisequantification.
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"scDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
CatalogNo. | GK814 |
CountryofManufacture | UnitedStates |
ProductCode | GQP-VIM2P |
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