Leukemia Inhibitory Factor, recombinant human 重组人白血病抑制因子

• LeukemiaInhibitoryFactor,recombinanthuman

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  Description: LeukemiaInhibitoryFactor,recombinanthuman

  TradeName:Chemicon(Millipore)
   
  ProductOverview:LeukemiaInhibitoryFactor(LIF)isalymphoidfactorwhichpromoteslong-termmaintenanceofembryonicstemcellsbysuppressingspontaneousdifferentiation.LIFhasanumberofotheractivitiesincludingcholinergicneurondifferentiation,controlofstemcellpluripotency,boneandfatmetabolism,mitogenesisofcertainfactordependentcelllinesandpromotionofmegakaryocyteproductioninvivo.HumanLIFisa19.7kDaproteincontaining181aminoacidresidues.Thenon-glycosylated,E.coliexpressed,recombinanthumanLIFisindistinguishablefromnativeLIFinitsBIOLOGicalactivitiesinvitro.HumanandmurinematureLIFexhibita78%sequenceidentityattheaminoacidcontrol.HumanLIFisequallyactiveonbothhumanandmousecells.MurineLIFisapproximately1000foldlessactiveonhumancells,thanhLIF.
   
  KeyApplications:CellCulture
   
  ApplicationNotes:RECOMMENDEDQCPROTOCOL
  
  M1Bioassay
  
  1.TheM1bioassayisperformedusinginvitrosemi-solidagarcultures,whichcontainapproximately100cellsin1mLvolumesofDMEcontaining20%FCSin0.3%agar.
  
  2.Add100μLofsampleorhLIF(10(E4)units/mLin5%FCSinisotonicsaline)intwo-foldserialdilutionsinduplicateto35mmpetridishes.
  
  3.Add100μLof5%FCSinisotonicsalinetotwocontrolslides.
  
  4.Incubateat37°Cinfullyhumidifiedatmosphereof10%CO2inairfor7days.
  
  5.Scorethenumberofcoloniesthatshowdifferentiation(note:50unitsisdefinedastheamountofactivitywhichresultsin50%ofthecoloniesbeingdifferentiated).
  
  Visitwww.esgro-lif.comforadditionalinformation
  
  ManufacturedbyCHEMICONInternational,Inc.LIFisprotectedunderUSPatentnos.5,187,077,5,427,925,5,443,825,5,750,654and6,261,548,EuropeanPatentno.0285448andrelatedforeignpatentsandisnotavailableforresale.
   
  UsageStatement:Unlessotherwisestatedinourcatalogorothercompanydocumentationaccompanyingtheproduct(s),ourproductsareintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.
   
  Species:Human
   
  StemCellType:
  • HumanEmbryonicStemCells
  • NeuralStemCells
  • HematopoieticStemCells
  • MesenchymalStemCells
  • InducedPluripotentStemCells
   
  StemCellWorkflowStage:
  • Maintenance&Expansion
  • Differentiation
  • GeneralCellCulture
   
  Source:mLIFisexpressedinE.coliasafusionproteinwithGSTusingthepGEXexpressionsystem,cleavedfromGSTmoeitywiththrombinandpurifiedbyHPLCchromatography.
   
  Presentation:LiquidinPBS,pH7.4and0.02%Tween20.Nopreservativiesadded.
   
  StorageConditions:Maintainat2-8°Cfor6-12months.Furtherdilutionsshouldbemadeintobufferormediumtowhichprotein(e.g.,1%BSA)orTween20hasbeenadded.
  
  ManufacturedbyCHEMICONInternational,Inc.LIFisprotectedunderUSPatentnos.5,187,077,5,427,925,5,443,825,5,750,654and6,261,548,EuropeanPatentno.0285448andrelatedforeignpatentsandisnotavailableforresale.
   
  SpecificActivity:
  TheactivityofhumanLIFisdeterminedbytheabilitytoinducedifferentiationofM1myeloidleukemiccells.TheminimumdetectableconcentrationofhumanLIFinthisassayis0.5ng/mL.Thespecificactivityis>/=1x10(E8)units/mg,where50unitsisdefinedastheamountofhumanLIFrequiredtoinducedifferentiationin50%oftheM1coloniesin1mLagarcultures.
   
  CellCultureReagentType:
  Cytokines&GrowthFactors
   
  GeneSymbol:
  • LIF
  • Emfilermin
  • MLPLI
  • HILDA
  • D-FACTOR
  • CDF
   
  ProteinorEnzymeType:Cytokines&GrowthFactors
   
  AlternateNames:LIF
   
  Concentration:10μg/mL
   
  Purity:Greaterthan95%byanalyticalHPLCandSDS-PAGE.Endotoxinlevelislessthan0.1ngpermgofLIF.Testednegativeinbothasepticandmicroplasmictests.

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