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Prosci/Acrp30 Recombinant Protein/40-327/0.005 mg

Specifications

SPECIES:Human
SOURCESPECIES:E.coli
SEQUENCE:MKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTN
TESTEDAPPLICATIONS:
BIOLOGICALACTIVITY:BiologicalactivitywasdeterminedbyitsABIlitytoinhibittheproliferationofmurineM1cells.TheexpectedED50forthiseffectis1.0-3.0μg/mL.

Properties

PURITY:Greaterthan98%bySDS-PAGEgelandHPLCanalyses.

Endotoxinlevelislessthan0.1ngperμg(1EU/μg).
PHYSICALSTATE:Lyophilized
STORAGECONDITIONS:ThelyophilizedAcrp30recombinantproteinisstableforatleast2yearsfromdateofreceiptat-20˚C.ReconstitutedAcrp30isstableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20˚C.Aswithanyprotein,exposingAcrp30recombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature.

AdditionalInfo

ALTERNATENAMES:ACDC,ADPN,APM1,APM-1,GBP28,ACRP30,ADIPQTL1,ACDC,Adiponectin,30kDaadipocytecomplement-relatedprotein
ACCESSIONNO.:NP_001171271.1
PROTEINGINO.:295317372

Background

gAcrp30isanaturallyoccurringglobularprotein,obtainedbyproteolyticprocessingofadiponectin.Adiponectinisproducedandsecretedexclusivelybyadipocytes,andisarelativelyabundantplasmaprotein,accountingforupto0.05%oftotalserumprotein.LikeAdiponectin,gAcrp30iscapableofdecreasinghyperglycemiaandreversinginsulinresistance.Additionally,gAcrp30hasbeenshowntobeanimportantfactorinpromotingfatlossbysignalingmuscletoabsorbandburnFree-FattyAcids(FFAs).ThesignalingreceptorsforadiponectinandgAcrp30haverecentlybeenidentifiedandnamesAdipoR1andAdipoR2.AdipoR2ispredominantlyexpressedintheliver.RecombinanthumangAcrp30/Adipoleanisa16.6kDaproteinconsistingof145aminoacidresidues.
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