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| SPECIES: | Mouse |
|---|---|
| SOURCE SPECIES: | HEK293 cells |
| SEQUENCE: | Val 38 - Lys 245 |
| FUSION TAG: | His Tag |
| TESTED APPLICATIONS: | ELISA, WB |
| APPLICATIONS: | This recombinant protein can be used for E, WB. For research use only. |
| BIOLOGICAL ACTIVITY: | Measured by its binding ability in a functional ELISA. Immobilized Mouse B7-1, His Tag at 2 ug/mL (100 uL/well) can bind Mouse CTLA-4, Fc Tag with a linear range of 0.12-0.5 ng/mL (QC tested). |
| PURITY: | >95% as determined by SDS-PAGE. Endotoxin level is less than 1.0 EU per ug by the LAL method. |
|---|---|
| PREDICTED MOLECULAR WEIGHT: | 25.6 kDa |
| PHYSICAL STATE: | Lyophilized |
| BUFFER: | PBS, pH7.4 |
| STORAGE CONDITIONS: | Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles. |
| ALTERNATE NAMES: | CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7 |
|---|---|
| ACCESSION NO.: | Q00609-1 |
| B7-1 and B7-2, together with their receptors CD28 and CTLA4, constitute one of the dominant co-stimulatory pathways that regulate T and Bcell responses. Although both CTLA4 and CD28 can bind to the same ligands, CTLA4 binds to B71 and B72 with a 20 100 fold higher affinity than CD28 and is involved in the downregulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-kappaB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas. |
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