LOCATION: | Clupeptideisa14aminoacidsyntheticpeptideneartheinternalregionofClu(aa312-325)(mouse). |
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TESTEDAPPLICATIONS: | Blocking |
APPLICATIONS: | ABCB5PeptideisusedforblockingtheactivityofABCB5antibody(catalognumber42-892).TheABCB5antibodyshipsat0.5mg/mLandtheABCB5Peptideshipsasa100μgpellet.Whenpeptideisreconstitutedin200μLwater,theconcentrationwillbe0.5mg/mL,identicaltothatoftheantibody.Thebeststartingratiowouldbe1:1(whichmeansmolarexcessofpeptiderelativetoantibodywhenidenticalvolumesaremixed).Mixequalvolumeofpeptideandantibodyattherequireddilutionandleaveatambienttemperaturefor20minutes.Thenincubateanddevelopinparalleltwoblots:onewiththeantibodyandonewiththeantibodywhichhasbeenpre-absorbedwiththeprovidedpeptide. |
PHYSICALSTATE: | Lyophilized |
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BUFFER: | ABCB5peptideissuppliedas100μgofdriedpeptide.Whenthepeptideisreconstitutedin200μLwater,theconcentrationwillbe0.5mg/mL. |
STORAGECONDITIONS: | Storeat-20˚C.Whenreconstitutedminimizefreezingandthawing. |
ALTERNATENAMES: | ABCB5beta,EST422562,ABCB5alpha,ATP-bindingcassettesub-familyBmember5,ABCB5P-gp, |
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ACCESSIONNO.: | NP_001157413.1,NP_848654.3,NP_001157414.1,NP_001157465.1 |
PROTEINGINO.: | 255708477 |
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