LOCATION: | AIF1peptidecorrespondsto15aminoacidsneartheintermediatedomainofAIF1. |
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SPECIES: | Human,Mouse |
TESTEDAPPLICATIONS: | Blocking |
APPLICATIONS: | AIF1PeptideisusedforblockingtheactivityofAIF1antibody(catalognumber45-220).TheAIF1antibodyshipsat0.5mg/mLandtheAIF1Peptideshipsasa100ugpellet.Whenpeptideisreconstitutedin200uLwater,theconcentrationwillbe0.5mg/mL,identicaltothatoftheantibody.Thebeststartingratiowouldbe1:1(whichmeansmolarexcessofpeptiderelativetoantibodywhenidenticalvolumesaremixed).Mixequalvolumeofpeptideandantibodyattherequireddilutionandleaveatambienttemperaturefor20minutes.Thenincubateanddevelopinparalleltwoblots:onewiththeantibodyandonewiththeantibodywhichhasbeenpre-absorbedwiththeprovidedpeptide. |
PHYSICALSTATE: | Lyophilized |
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BUFFER: | AIF1peptideissuppliedas100μgofdriedpeptide.Whenthepeptideisreconstitutedin200μLwater,theconcentrationwillbe0.5mg/mL. |
STORAGECONDITIONS: | Storeat-20˚C.Whenreconstitutedminimizefreezingandthawing. |
ALTERNATENAMES: | IBA1,IRT1,AIF-1,IRT-1,G1,IBA1,Allograftinflammatoryfactor1,Ionizedcalcium-bindingadaptermolecule1 |
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ACCESSIONNO.: | NP_001614.3 |
PROTEINGINO.: | 14574568 |
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