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Roche11684795910原位细胞凋亡检测试剂盒,荧光素标记,Tunnel检测

InSituCellDeathDetectionKit,Fluorescein

Kitforthedetectionandquantificationofapoptosis(programmedcelldeath)atthesingle-celllevel,basedonlabelingofDNAstrandbreaks(TUNELtechnology):Analysisbyfluorescencemicroscopyorflowcytometry

Application

Qualitativedetectionofapoptosisatthesingle-celllevelbyfluorescencemicroscopyandquantitativedetectionbyflowcytometry.

Benefits

Sensitive:Thedirectlabelingprocedureusingfluorescein-dUTPreducesbackgroundlabeling
Fast:Theuseoffluorescein-dUTPallowsanalysisofthesamplesdirectlyaftertheTUNELreaction
Convenient:Nosecondarydetectionsystemrequired
Accurate:Identificationofapoptosisatamolecularlevel(DNA-strandbreaks)andidentificationofcellsattheveryearlystagesofapoptosis

ProductDescription

Samplematerial:CellsinsUSPension,cytospinandcellsmearpreparations,adherentcellsgrownonslides,andfrozenandparaffin-embeddedtissuesections.

BackgroundInformation

WidelyusedmethodstodetermineapoptosisincludetheanalysisofthegenomicDNAbyagarose-gelelectrophoresisandDNAfragmentationassaysbasedon³H-thymidineand,alternatively,5-Bromo-2-deoxy-uridine.Themethodsinvolvetheseparationoffragmented,lowmolecularweightDNAfromunfragmented,highmolecularweightDNAinagivencellpopulation.Thus,thesemethodsdonotprovideinformationaboutthefateofanindividualcellinagivencellpopulation,orparticularly,intissuesections.Alternatively,individualapoptoticcellsmaybemicroscopicallyrecognizedbecauseofthecharacteristicappearanceofnuclearchromatincondensationandfragmentation,butthismethodissubjectiveandlimitedtoarelativelynarrowtimewindowwhenthemorphologicalchangesareatamaximum.
ThehallmarkofapoptosisisDNAdegradation,whichinearlystages,isselectivetotheinternucleosomalDNAlinkerregions.TheDNAcleavagemayyielddouble-strandedandsingle-strandedDNAbreaks(nicks).Bothtypesofbreakscanbedetectedbylabelingthefree3-OHterminiwithmodifiednucleotides(e.g.,biotin-dUTP,DIG-dUTP,fluorescein-dUTP)inanenzymaticreaction.Theenzymeterminaldeoxynucleotidyltransferase(TdT)catalyzesthetemplate-independentpolymerizationofdeoxyribonucleotidestothe3-endofsingle-anddouble-strandedDNA.ThismethodhasalsobeentermedTUNEL(TdT-mediateddUTP-Xnickendlabeling).Alternatively,free3-OHgroupsmaybelabeledusingDNApolymerasesbythetemplate-dependentmechanismcallednicktranslation.However,theTUNELmethodisconsideredtobemoresensitiveandfaster.

Contents

EnzymeSolution(TdT),5vials
LabelSolution(fluorescein-dUTP),5vials

Principle

TheInSituCellDeathDetectionKitFluorescein isbasedonthedetectionofsingle-anddouble-strandedDNAbreaksthatoccurattheearlystagesofapoptosis.
ApoptoticcellsarefixedandpermeABIlized.Subsequently,thecellsareincubatedwiththeTUNELreactionmixturethatcontainsTdTandfluorescein-dUTP.Duringthisincubationperiod,TdTcatalyzestheadditionoffluorescein-dUTPatfree3-OHgroupsinsingle-anddouble-strandedDNA.Afterwashing,thelabelincorporatedatthedamagedsitesoftheDNAisvisualizedbyflowcytometryand/orfluorescencemicroscopy.

Figure1:SchematicshowingtheprincipleoftheInSituCellDeathDetectionKits,FluoresceinandTMRred.

订购指南：以下产品部分现货供应

检测方法
产品名称
产品货号
规格
价格￥
FACS
Fluorescence
orlight
microscopies
Annexin-V-FLUOS
11828681001
250T
5023
Annexin-V-Alexa568
03703126001
250T
5443
Annexin-V-Biotin
11828690001
250T
5023
Annexin-V-FLUOSStainingKit
11858777001
50T
1932
Annexin-V-FLUOSStainingKit
11988549001
250T
6233
ELISA
Caspase3ActivityAssay
12012952001
96T
6578
CellDeathDetectionELISA^PLUS
11774425001　
96T
3814
TUNEL
InSituCellDeathDetectionKit
Fluorescein
11684795910
50T
4662
InSituCellDeathDetectionKit
AP
11684817910
50T
5267
InSituCellDeathDetectionKit
POD
11684817910
50T
5267
InSituCellDeathDetectionKit
TMRred
12156792910
50T
3931

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技术文章

检测方法	产品名称	产品货号	规格	价格￥
FACS Fluorescence orlight microscopies	Annexin-V-FLUOS	11828681001	250T	5023
	Annexin-V-Alexa568	03703126001	250T	5443
	Annexin-V-Biotin	11828690001	250T	5023
	Annexin-V-FLUOSStainingKit	11858777001	50T	1932
	Annexin-V-FLUOSStainingKit	11988549001	250T	6233
ELISA	Caspase3ActivityAssay	12012952001	96T	6578
ELISA	CellDeathDetectionELISA^PLUS	11774425001	96T	3814
TUNEL	InSituCellDeathDetectionKit Fluorescein	11684795910	50T	4662
	InSituCellDeathDetectionKit AP	11684817910	50T	5267
	InSituCellDeathDetectionKit POD	11684817910	50T	5267
	InSituCellDeathDetectionKit TMRred	12156792910	50T	3931