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In Situ Cell Death Detection Kit, POD 过氧化物酶标记的原位细胞凋亡检测试剂盒188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品

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In Situ Cell Death Detection Kit, POD 过氧化物酶标记的原位细胞凋亡检测试剂盒

InSituCellDeathDetectionKit,POD

Kitforthe detectionandquantificationofapoptoticcelldeathonasingle-celllevelbylightmicroscopyinimmunocyto-andimmunohistochemistry

Application

Qualitativedetectionofapoptosisatthesingle-celllevelbylightmicroscopy.

Benefits

Sensitive:Themaximumintensityoflabeling(cellstaining)ofapoptoticcellsishigherthanthenicktranslationmethod
Fast:Theuseoffluorescein-dUTPallowsanalysisofthesamplesdirectlyaftertheTUNELreaction,butbeforetheadditionofthesecondarydetectionsystem
Convenient:Thedirectlabelingprocedureusingfluorescein-dUTPallowsverificationoftheefficiencyoftheTUNELreactionduringtheassayprocedure
Accurate:Identificationofapoptosisatamolecularlevel(DNA-strandbreaks)andidentificationofcellsattheveryearlystagesofapoptosis
FlexIBLe:Nosubstrateincluded;providestheopportunitytoselectthestainingprocedureofchoice

ProductDescription

Samplematerial:Cytospinandcellsmearpreparations,adherentcellsgrownonslides,andfrozenandparaffin-embeddedtissuesections.

BackgroundInformation

WidelyusedmethodstodetermineapoptosisincludetheanalysisofthegenomicDNAbyagarose-gelelectrophoresisandDNAfragmentationassaysbasedon³H-thymidineand,alternatively,5-Bromo-2-deoxy-uridine.Themethodsinvolvetheseparationoffragmented,lowmolecular-weightDNAfromunfragmented,highmolecular-weightDNAinagivencellpopulation.Thus,thesemethodsdonotprovideinformationaboutthefateofanindividualcellinagivencellpopulationor,particularly,intissuesections.Alternatively,individualapoptoticcellsmaybemicroscopicallyrecognizedbecauseofthecharacteristicappearanceofnuclearchromatincondensationandfragmentation,butthismethodissubjectiveandlimitedtoarelativelynarrowtimewindowwhenthemorphologicalchangesareatamaximum.
ThehallmarkofapoptosisisDNAdegradation,whichinearlystagesisselectivetotheinternucleosomalDNAlinkerregions.TheDNAcleavagemayyielddouble-strandedandsingle-strandedDNAbreaks(nicks).Bothtypesofbreakscanbedetectedbylabelingthefree3-OHterminiwithmodifiednucleotides(e.g.,biotin-dUTP,DIG-dUTP,fluorescein-dUTP)inanenzymaticreaction.Theenzymeterminaldeoxynucleotidyltransferase(TdT)catalyzesthetemplate-independentpolymerizationofdeoxyribonucleotidestothe3-endofsingle-anddouble-strandedDNA.ThismethodhasalsobeentermedTUNEL(TdT-mediateddUTP-Xnickendlabeling).Alternatively,free3-OHgroupsmaybelabeledusingDNApolymerasesbythetemplate-dependentmechanismcallednicktranslation.However,theTUNELmethodisconsideredtobemoresensitiveandfaster.

Contents

EnzymeSolution(TdT),5vials
LabelSolution(fluorescein-dUTP),5vials
ConverterPOD(anti-fluoresceinantibody-POD),ready-to-use

Principle

TheInSituCellDeathDetectionKit,POD isbasedonthedetectionofsingle-anddouble-strandedDNAbreaksthatoccurattheearlystagesofapoptosis.
ApoptoticcellsarefixedandpermeABIlized.Subsequently,thecellsareincubatedwiththeTUNELreactionmixturethatcontainsTdTandfluorescein-dUTP.Duringthisincubationperiod,TdTcatalyzestheadditionoffluorescein-dUTPatfree3-OHgroupsinsingle-anddouble-strandedDNA.Afterwashing,thelabelincorporatedatthedamagedsitesoftheDNAismarkedbyananti-fluoresceinantibodyconjugatedwiththereporterenzymeperoxidase.Afterwashingtoremoveunboundenzymeconjugate,thePODretainedintheimmunecomplexisvisualizedbyasubstratereaction.

Figure1:Testprinciple.

订购指南：以下产品部分现货供应

检测方法	产品名称	产品货号	规格	价格￥
FACS Fluorescence orlight microscopies	Annexin-V-FLUOS	11828681001	250T	5023
	Annexin-V-Alexa568	03703126001	250T	5443
	Annexin-V-Biotin	11828690001	250T	5023
	Annexin-V-FLUOSStainingKit	11858777001	50T	1932
	Annexin-V-FLUOSStainingKit	11988549001	250T	6233
ELISA	Caspase3ActivityAssay	12012952001	96T	6578
ELISA	CellDeathDetectionELISA^PLUS	11774425001	96T	3814
TUNEL	InSituCellDeathDetectionKit,Fluorescein	11684795910	50T	4662
	InSituCellDeathDetectionKit,AP	11684809910	50T	5267
	InSituCellDeathDetectionKit,POD	11684817910	50T	5267
	InSituCellDeathDetectionKit,TMRred	12156792910	50T	3931

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