AnovelbiosensorbasedoncompetitiveSERSimmunoassayandmagneticseparationforaccurateandsensitivedetectionofchloramphenicol
Kang Yang, Yongjun Hu , Ning Dong
Abstract
Theaccurateandsensitivedetectionofchloramphenicol(CAP)isparticularlyimperativetopublichealthandsafety.Here,wepresentanovelsensorforresidualCAPdetectionbasedoncompetitivesurface-enhancedRamanscattering(SERS)immunoassayandmagneticseparation.Inthisnanosensor,functionalizedAunanoparticles(AuNPs)werelabeledwiththeRamanreportermolecule(e.g.4,4′-dipyridyl).WiththeadditionoffreeCAP,acompetitiveimmunereactionwasinitiatedbetweenfreeCAPandaboveAuNPsforconjugatingwithCAPantibody-modifiedmagneticnanoparticles(MNPs).Insteadofthesolidsubstrate,theantibodyconjugated-magneticbeadswereusedassupportingmaterialsandseparationtoolsinthepresentsensor.Withtheaidofamagnet,themixturewasremovedfromthesupernatantforconcentrationeffects.ThiscausedobviouschangeofSERSsignalintensityobtainedfromsupernatant.TheSERSsignalswerecollectedfromthesupernatantdirectly,whichmadetheSERSmeasurementsmorestable,repeatableandreliable.TheproposedSERS-basedmagneticimmunosensorallowsustodetectCAPinafast,selectiveandsensitive(1.0 pg/mL)manneroverawideconcentrationrange(1–1×10
4 pg/mL).Inaddition,theseresultsdemonstratethatthisimmunosensorholdsgreatpotentialforthe
detectionofantibioticsinrealaquaticenvironment,whichiscrucialtoourlife.
Keywords
- Chloramphenicol;
- Surface-enhancedRamanscattering;
- Competitive;
- Magneticseparation
CAP antibody, CAP-BSA and CAP standard so-lution were purchased from Otwo Biotech (Shenzhen) Inc.
CAPAntibody-产品
Cat#：PL0306284
Isotype:RabbitPolyclonalIgG
Immunogen：PeptidederivedfromhumanBrd4/CAP.
Purification: Immunogenaffinitypurified
StorageBuffer: PBS,pH7.4(1%BSAand0.1%Sodiumazide)
Background：
Brd4belongstotheBETfamily,agroupofstructurallyrelatedproteinscontainingtwobromodomains.Throughthesetwodomains,Brd4associateswithmitoticchromosomesanditsexpressioncorrelateswithcellgrowth.ExpressionofBrd4inhibitscellcycleprogressionfromG(1)toS,duetobindingtothelargestsubunitofreplicationfactorC(RFC)topreventDNAelongation.
AlternateNames：
Brd4;BRD4_HUMAN;bromodomaincontainingprotein4;Bromodomain-containingprotein4;CAP;chromosomeassociatedprotein;HUNK1;ProteinHUNK1.
SpecificityReaction：Hu,Mo,Rat,Chi,Dog,Pig,Cow,Hor,
ApplicationNotes:WB:1:100-500E:1:500-1000IHC:1:100-500IF:1:50-200
Storage：
Storeat–20ºCforoneyear.Avoidrepeatedfreeze-thawcycles.At4ºCfor2-3weeksafterreconstituted.
产品文献：相关链接里面的附件
A novel biosensor based on competitive SERS immunoassay and magnetic separation for accurate and sensitive detection of chloramphenicolKang Yang, Yongjun Hu , Ning Dong Biosensors and Bioelectronics 80 (2016) 373–377
Thisproductforresearchuseonly,notforuseinhumans,therapeuticordiagnosticprocedures.
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