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Exocell/Universal TBARs/1020

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UniversalTBARs

Manufacturer/Trade:Exocell/UniversalTBARS
CatalogueNumbers:1020
Methodology:ChemicalAssay
Summaryofprocedure:

TheTBARsassaykitmeasuresmalondialdehyde(MDA),areactivecompoundformedfromlipidperoxidesthataregeneratedunderconditionsofoxidativestress.Oxidativemodificationoflipidsoccurswithagingandvariousdiseases,andincreasedoxidativestressisassociatedwithdiabetesanditscomplications.Cellularexposuretooxidativestressgivesrisetohighlyreactiveandunstablelipidhydroperoxides.DecompositionofunstableperoxidesderivedfrompolyunsaturatedfattyacidsleadstotheMDAformation.MDAreactswiththiobarbituricacid(TBA)toformanadductthatcanbemeasuredspectrophotometricallyorfluorometrically,thelatterprovidinggreatersensitivity.DeterminationofTBARsinBIOLOGicalsampleshasbecomethepreferredmethodforassessinglipidperoxidationandoxidativestress.Exocell"sTBARsassaycanbeusedwithaspectrumofbiologicalsamplesincludingbodyfluids,tissueandcellspecimens.

Tocompletetheassay,standardorsampleisreactedwiththiobarbituricacidandheatedinthepresenceofaceticacid.Aftercoolingandclarificationbycentrifugation,aliquotsareplacedintomicrotiterwellsofablackplateandreadinafluorometeratexcitation484nm,emission530nm,orintoaplasticplateforreADIngat530-540nminaspectrophotometer.MDAconcentrationinsamplesisdeterminedfromthestandardcurve.

SpecimenRequired:100uL
AssayRange:0-30nmolefluorometrically
0-300nmolespectrophotometrically
Precision:Intra-andinter-assayprecision<8%



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