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ApolipoproteinB

Manufacturer/Trade:Exocell/ApoBTest
CatalogueNumbers:1006StripPlate
Methodology:IndirectCompetitiveELISA
Summaryofprocedure:TheApoBTestisdesignedforthequantitativemeasurementofapolipoproteinBinhumanplasma.Tocompletetheassay,B36monoclonalantibodyisaddedtoamicrotiterplatewellthatcontainsapolipoproteinBimmobilizedtothesolidphase,andtowhichapolipoproteinB,standardorsample,isaddedinthefluidphase.Duringa30minuteincubation,themonoclonalantibodybindstoeithertheimmobilizedapoBortothesolubleapoB,hencethenotionofcompetitivebinding.Unboundreactantsarethenwashedaway,andasecondincubationof15minuteswithanenzymeconjugatedtoananti-mouseantibodyreactswiththeB36thatwascapturedbythesolidphase.Thewellsarewashedtoremoveunboundsecondaryantibody,andTMBSubstratesolutionisaddedtodetectboundconjugate.Thechromogenicreactionisstoppedbyanadditionofdiluteacid,andcolorintensitydeterminedbyabsorbanceat450nm.Sampleconcentrationisdeterminedbyrelatingresponsetothatforknownstandards.
SpecimenRequired:10ulcitrateorEDTAtreatedplasma
AssayRange:0.13-2.0mg/ml(correspondingto13-200mg/dl)
Precision:Intra-andinterassayprecisionofsampleswithintheassayrangehaveaC.V.<10%.
Specificity:ThemonoclonalantibodyB36isspecificforhumanapolipoproteinB,anddoesnotcross-reactwithotherproteinsorsubstancesfoundinhumanplasma.
ExpectedValues:ExpectedvaluesforapoBinmalesubjectsarereportedtobe108+/-33mg/dlforindividualsfreeofcoronaryarterydisease(CAD),and131+/-37forsubjectswithangiographicallydocumentedprematureCAD.Valuesforwomenaresimilartothoseformen.



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