产品资料

Abcam/Recombinant human GRP78 BiP protein (Active) (ab78432)/ab78432

Product name
Recombinant human GRP78 BiP protein (Active)See all GRP78 BiP proteins and peptides
Biological activity
ab78432 has ATPase activity at the time of manufacture of 2.3µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP, using a Malachite Green assay.
Purity
>90% SDS-PAGE.Ab78432 is purified by affinity purification.
Expression system
Escherichia coli
Accession
P11021
Protein length
Full length protein
Animal free
No
Nature
Recombinant
- Species
  Human
- Sequence
  MKLSLVAAML LLLSAARAEE EDKKEDVGTV VGIDLGTTYS CVGVFKNGRV EIIANDQGNR ITPSYVAFTP EGERLIGDAA KNQLTSNPEN TVFDAKRLIG RTWNDPSVQQ DIKFLPFKVV EKKTKPYIQV DIGGGQTKTF APEEISAMVL TKMKETAEAY LGKKVTHAVV TVPAYFNDAQ RQATKDAGTI AGLNVMRIIN EPTAAAIAYG LDKREGEKNI LVFDLGGGTF DVSLLTIDNG VFEVVATNGD THLGGEDFDQ RVMEHFIKLY KKKTGKDVRK DNRAVQKLRR EVEKAKRALS SQHQARIEIE SFYEGEDFSE TLTRAKFEEL NMDLFRSTMK PVQKVLEDSD LKKSDIDEIV LVGGSTRIPK IQQLVKEFFN GKEPSRGINP DEAVAYGAAV QAGVLSGDQD TGDLVLLDVC PLTLGIETVG GVMTKLIPRN TVVPTKKSQI FSTASDNQPT VTIKVYEGER PLTKDNHLLG TFDLTGIPPA PRGVPQIEVT FEIDVNGILR VTAEDKGTGN KNKITITNDQ NRLTPEEIER MVNDAEKFAE EDKKLKERID TRNELESYAY SLKNQIGDKE KLGGKLSSED KETMEKAVEE KIEWLESHQD ADIEDFKAKK KELEEIVQPI ISKLYGSAGP PPTGEEDTAE KDEL
- Predicted molecular weight
  78 kDa
- Tags
  His tag N-Terminus
- Additional sequence information
  his-tagged(Gene ID 3309)(NM_005347)

Related Products
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-GRP78 BiP antibody (ab21685)
- Anti-GRP78 BiP antibody (ab32618)
- Anti-6X His tag® antibody [4D11] (ab5000)

Our Abpromise guarantee covers the use ofab78432in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Stability and Storage
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
pH: 7.40Constituents: 0.61% Tris, 0.87% Sodium chloride, 10% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.

Alternative names
- 78 kDa glucose regulated protein
- 78 kDa glucose-regulated protein
- AL022860
- AU019543
- BIP
- D2Wsu141e
- D2Wsu17e
- Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
- Endoplasmic reticulum lumenal Ca2+ binding protein grp78
- Epididymis secretory sperm binding protein Li 89n
- FLJ26106
- Glucose Regulated Protein 78
- Glucose Regulated Protein 78kDa
- GRP 78
- GRP-78
- GRP78
- GRP78_HUMAN
- Heat shock 70 kDa protein 5
- Heat Shock 70kDa Protein 5
- Heat shock protein family A (Hsp70) member 5
- HEL S 89n
- Hsce70
- HSPA 5
- HSPA5
- Immunoglobulin Heavy Chain Binding Protein
- Immunoglobulin heavy chain-binding protein
- mBiP
- MIF2
- Sez7
see all
Function
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Involvement in disease
Autoantigen in rheumatoid arthritis.
Sequence similarities
Belongs to the heat shock protein 70 family.
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP11021The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).
Information by UniProt

Description