产品资料

Abcam//ab78427

Product name
Recombinant human Hsp70 proteinSee all Hsp70 proteins and peptides
Biological activity
ab78427 has ATPase activity at the time of manufacture of 3.3µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH7.5) in thepresence of 20ul of 1mM ATP using a Malachite Green assay.
Purity
>90% SDS-PAGE.ab78427 is affinity purified.
Expression system
Escherichia coli
Accession
NM_005345
Protein length
Full length protein
Animal free
No
Nature
Recombinant
- Species
  Human
- Sequence
  MAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDK PKVQVSYKGE TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT KDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQ EAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEV D
- Tags
  His tag N-Terminus

Our Abpromise guarantee covers the use ofab78427in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Stability and Storage
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
pH: 7.50Preservative: 1.36% ImidazoleConstituents: 0.87% Sodium chloride, 10% Glycerol, 0.328% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.

Alternative names
- DnaK type molecular chaperone HSP70 1
- Epididymis secretory protein Li 103
- FLJ54303
- FLJ54370
- FLJ54392
- FLJ54408
- FLJ75127
- Heat shock 70 kDa protein 1
- Heat shock 70 kDa protein 1/2
- Heat shock 70 kDa protein 1A
- Heat shock 70 kDa protein 1A/1B
- Heat shock 70kDa protein 1B
- Heat shock induced protein
- HEL S 103
- HSP70 1
- HSP70 1A
- HSP70 1B
- HSP70 2
- HSP70-1
- HSP70-1/HSP70-2
- HSP70-1A
- HSP70-2
- HSP70.1
- HSP70.1/HSP70.2
- HSP71_HUMAN
- HSP72
- HSPA1
- HSPA1A
- HSPA1B
see all
Relevance
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.Tissue specificity: HSPA1B is testis-specific.
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Description