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| Description | One tube, one reaction – No stopper solution or multiple handling steps. Reverse transcribe RNA to high-quality cDNA, including removal of contaminating genomic DNA, in under 30 minutes using 5X All-In-One RT MasterMix with AccuRT. |
|---|---|
| SKU | G592 |
| Reactions | 100 rxns |
| dNTP or dUTP Based | dNTP Based |
| Applications | abm’s 5X All-in-One RT MasterMix with AccuRT offers a convenient, complete system for eliminating contaminating gDNA in RNA samples and rapid synthesis of ready-to-use cDNA for downstream applications. Key Features • Efficient digestion of ss/dsDNA• Maximal RNA protection – No harsh extraction steps required• One tube, one reaction – No stopper solution or multiple handling steps• Streamlined protocol suitable for high-throughput applications• Simple setup for any RNA template• High reproducibility and cDNA yield Note: For no-RT control, use a small aliquot of 5X All-In-One RT MasterMix with AccuRT (5 μl per no-RT sample you wish to include) and heat-inactivate all enzymes in the mastermix at 95°C for 5 min. |
| Storage Condition | Store all components at -25°C to -15°C in a non-defrost cycle freezer. All components are stable for up to one (1) year from the date of shipping, if stored and handled properly. |
| Unit quantity | 100 reactions x 20 μl |
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